Subject: Which Tenascin gene?
Dear S. Baumgartner,
I am a curator of FlyBase working at the Cambridge, England, site with
Prof. M. Ashburner.
I am curating the abstracts from the fifth European meeting on the
neurogenetics of Drosophila. Your abstract on page 18 discusses PCRs
derived from EGF-like region of tenascin.
Which tenascin gene are you using?
This information is vital for completion of the abstract curation so
your help would be invaluable.
Many thanks,
Eleanor Whitfield.
Subject: Which Tenascin gene?
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Dear Eleanor,
The primers were used from chicken tenascin, but since neurexin (nrx) has
EGF-like repeats as well, therefore, it is considered an accidential
amplification. The nrx gene resides at 68F3-6, gives a transcript of 5.0 kb, and
encodes a protein of 1283 aa with an apparent MW of about 155 kD with a domain
structure almost identical to vertebrate neurexin.
Hope this helps to complete the abstract curation.
Regards Stefan