Subject: Help FlyBase - Mad Hi Bill, I have three questions concerning your paper: \*x FBrf0080380 == Sekelsky et al., 1995, Genetics 139(3): 1347--1358 1) This aberration: Df(2L)DTD62 2P3D;H7 3P2D is mentioned in Table 1 on pg1349. We do not have DTD62, is it a T(2;3), what are its breakpoints, do you have a reference for it? Any other information you can provide would be marvellous too! We have T(2;3)H7 but the breakpoints you list do not match those we have in the database, are you revising the breakpoints or citing breakpoints from a previous paper, if so which one? 2) This aberration: Dp(2;3)JS20 is mentioned in Figure 1 on pg 1350. We do not have this aberration in the database and yet you fail to give any details, please tell me as much as possible. Previous reference would be yummy! 3) Transcript: c24 is mentioned in Figure 5 on pg1353. Was just wondering if you have done more work on this transcript and possibly given it a gene symbol yet? One less anon in the database if you have. Many thanks, Nellie > Subject: Re: Help FlyBase - Mad Hi Nellie, Here's the information you requested. Bill ======================================================================== T(2;3)DTD62 == T(2;3) 23F ; 80F Progenitor chromosomes: 2 == dppd-ho 3 == unknown wild-type Mutagen: X ray Discoverer: Gelbart Recovered on the basis of pseudolinkage between chromosomes 2 and 3. Subsequently shown to disrupt transvection in T(2;3)DTD62, dppd-ho / dpphr4 individuals (Gelbart, unpublished). ======================================================================== T(2;3)HVD7 == T(2;3) 23D1-2 ; 80F Synonym: T(2;3)H7 Progenitor chromosomes: 2 == dppd-ho 3 == unknown wild-type Mutagen: X ray Discoverer: Gelbart Recovered as a heldout fly in an experiment to identify dpp transvection- disrupting rearrangements (DTDs). Upon retesting, did not display a DTD effect, and so it is given the suffix 'HVD' for the consonants in Harvard according to proper Bostonian pronunciation. Arose during the mutageneses described in Gelbart, 1982 (FBrf0038632). ======================================================================== Df(2L)DTD62-HVD7 == Ts(2R;3L)DTD62 + Ts(2L;3R)HVD7 New order: 2Lt - 23D | 80F - 3Rt 2Rt - 23F | 80F - 3Lt Deficient for: 23D1-2 ; 23F Deleted for: Mad Synonym: Df(2L)DTD62 2P3D;H7 3P2D Mutagen: Synthetic Creator: Sekelsky Progenitors: T(2;3)DTD62 T(2;3)HVD7 Produced by segregation in a fly initially heterozygous for T( 2:3 )DTD62 and T(2;3)HVD7. ======================================================================== Dp(2;3)JS20 == Dp(2;3) 23C ; 23D ; 94D1-2 uDp Citation: FBrf0070678, pages 75-76 Title Mothers against dpp: a gene required for decapentaplegic function in Drosophila melanogaster. Author(s) Sekelsky, J.J. Year 1993 Journal Ph.D. Thesis, Harvard University, MA Pages ix + 152pp Type thesis Flybase ID FBrf0070678 Progenitor chromosomes: 2 == dppd-ho Tp(1;2)TE23CD 3 == unknown wild-type Mutagen: gamma ray Discoverer: Sekelsky Recovered as a red-eyed fly among yellow-eyed z1 w11E4 ; dppd-ho Tp(1;2)TE23CD, w+ / dppd-ho Tp(1;2)TE23CD, w+ dp siblings. Red eyes are due to the unpaired w+ gene inserted into 94D1-2. The reciprocal deletion segregant, if it arose, was not recovered. ======================================================================== >Transcript: c24 >is mentioned in Figure 5 on pg1353. >Was just wondering if you have done more work on this transcript and >possibly given it a gene symbol yet? One less anon in the database if you >have. Sorry, I can't help you on this one. Not only do we have nothing new on c24, but if I play things right, we never will. Studying this gene at all was just a by-product of honing in on the Mad gene. So, unless you want me to call it one of these 'next door gene' cutesy names, like Almost Mad, which I think would be absurd, the best thing to do is leave it wallowing in anon-imity, and live with its naffiness until someone comes along and actually studies the blessed gene. ========================================================================
