Dear Rachel,
In my nomenclature for transgenic lines, 1010T2 and 1010T10 represent
different insertions (T2 and T10) of the same plasmid (pBD1010). These
numbers are therefore quite meaningless to anyone but me, but since I have
unfortunately distributed these stocks to several people using these
designations, I guess they will have to stick.
Thanks, and best wishes,
Barry
>I am curating the genetic information about these constructs which you
>kindly deposited with Kath in the stock center. To be sure we get this
>right, could you confirm that 1010T2 and 1010T10 are insertion
>identifiers, as opposed to a description of something that is inside the
>transposon? (or otherwise set us straight!)
>
>> 1010T2: UAS-GFPS65T, pCaSpeR3, II.chromosome homozygous viable
>> insert, construct and transgene done by Barry Dickson (unpublished)
>> using the GFPS65T mutant <up>Heim, R. and Tsien, R.Y. (1996) Current
>> Biology 6, 178-182</up>.
>>
>> 1010T10: UAS-GFPS65T, pCaSpeR3, III.chromosome homozygous viable
>> insert, construct and transgene done by Barry Dickson (unpublished)
>> using the GFPS65T mutant <up>Heim, R. and Tsien, R.Y. (1996) Current
>> Biology 6, 178-182</up>.
>
>with best wishes,
>
>Rachel
>