Mariani, B.D., Shea, M.J., Conboy, M.J., Conboy, I., King, D.L., Kafatos, F.C. (1996). Analysis of regulatory elements of the developmentally controlled chorion s15 promoter in transgenic Drosophila. Dev. Biol. 174(1): 115--124.
FlyBase ID
FBrf0086535
Publication Type
Research paper
Abstract
The Drosophila s15 chorion gene is expressed only in the follicular epithelium surrounding the developing oocyte, with tight quantitative control and a very narrow temporal specificity. We have used germ-line transformation analysis to conduct an extensive mutational dissection of its promoter between -189 and -39 bp relative to the transcriptional start site. Quantitative control and temporal specificity are disrupted by several of these mutations. The results suggest that this 150-bp DNA region encompasses many positive and negative, at least partially degenerate, cis-regulatory elements, which are involved in specifying the highly precise expression pattern of the s15 gene during development.
