Subject: sequence transposon pP{DM23}
Denise Clark sent the following comments to flybase-help:
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A sequence is inverted: i.e.the 7.2 kb HindIII rosy fragment
is inverted (1-7288).  The construct report states the
progenitor is assumed to be pP{Car30A}.  It must be
pP{Car30B} (Mismer and Rubin 1987 just say Carnegie 30).
I have two reasons to believe this:
1)SalI + XhoI digest gives 3 + 8 kb bands,
which is what you'd predict for a Car30B
derivative of DM23
2) I made a primer from the rosy sequence
and one from the fragment I inserted into the
mcs.  I got a PCR product which could only be
explained with a Car30B derivative.
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Remote IP address: 131.202.97.10
Dear Denise,
Thanks for the information concerning P{DM23}.  This is exactly the
kind of input we had hoped to get from the fly community.  Do you
have any objection to our retaining your e-mail message as a personal
communication that we can cite in the pP{DM23} and P{DM23} reports?
Thanks again for taking the time to contact us.
Sincerely,
Lynn Crosby
FlyBase
Dear Lynn,
I have no objection to you using my e-mail message.  Glad to be of help.
Its so nice to have all those vector sequences compiled!  No more cutting
and pasting from Genbank sequences to put it together ourselves!
Denise