Subject: Help FlyBase - ea5022rx1 Dear Dr. Anderson, I am a curator of FlyBase working at the Cambridge, England site with Prof. M. Ashburner. During curation I have come across ea5022rx1. In papers this is used as a ea- mutation (in combination with ea4). And in: FBrf0083893 == Erdelyi et al., 1995, Nature 377(6549): 524--527 is stated to fail to complement TmII mutations. With this information I would like to make Df(3R)ea5022rx1. Currently Df(3R)ea5022rx1 is a synonym under eaD10 (L&Zs symbol for 5022). Could you please tell me if ea5022rx1 is broken in ea or completely deletes ea? How is this mutation related to ea5022 (a point mutation) is it a revertant? I have traced a lot of this information via papers from R. Chasan and references to her PhD thesis, but unfortunately I only have a snail mail address for her. If you would like to pass this query on to her then please do so. Many thanks, Eleanor Whitfield Subject: Re: Help FlyBase - ea5022rx1 Dear Dr. Whitfield, ea5022RX1 is an X-ray induced revertant of ea5022 made by J. Szabad. ea5022RX1 completely deletes easter; it also appears to delete all the DNA in the clone lambaTMR16 from the Eric Fyrberg walk through the region, but we have not mapped its breakpoints. I hope this is helpful. Sincerely, Kathryn Anderson