Subject: helping FlyBase
Hi Allen,
I have a question about a marked balancer that you published in 1993. It
is the y+ CyO chromosome published in Timmons et al., 1993, Dev. Biol.
158(2): 364--379.
It was said to have been constructed by Elizabeth Woodhouse (Materials and
Methods, bottom of page 365).
Has this element been previously published? If so, can you tell me which
y+ construct was used? We have records for many y+ constructs in
FlyBase so its possible that we already have an entry for it, and I would
like to link this balancer to the correct construct, if at all possible.
If it is one that Elizabeth made herself, if you could let me know a couple
of details, such as size of yellow genomic DNA fragment, and transformation
vector, then that would be wonderful.
many thanks for your help,
with best wishes,
Rachel.
Date-Warning: Date header was inserted by jhmail.hcf.jhu.edu
Subject: Re: helping FlyBase
Dear Rachel,
The y+ construct in a P-element vector was made by Pam Geyer at The
University of Iowa. The insert we started with was on an otherwise unmarked
2nd chromosome. I am afraid that I do not know the details of the
construct. We have subsequently transposed the same insert onto a TM3
chromosome that is marked with Ser and e but not Sb. The y+ has been
completely stable on both balancers.
Sincerely,
Allen
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ALLEN SHEARN
Department of Biology Johns Hopkins University Baltimore, MD 21218