Subject: Re: \*x FBrf0086385 == Doherty et al., 1996, Genes Dev. 10(4): 421--434 Rachel, Here is the information you requested about the UAS nuclear lacZ construct. Let me know if you have any other questions. Take care, Dan >origin of nuclacZ: >vector D237 = Act5c>Draf+>nuc-lacZ from Gary Struhl (Struhl and Basler, >1992 Cell > 72:527-540 where he describes the FLP-out technique) >transformation vec: pUAST <up>white+ in flies and amp resistant in bacteria</up> >how many Gal4 binding sites:5 >what promoter: hsp70. >published before: no