Subject: Help FlyBase - larval touch insensitive lines Hi Dr. Kernan, I am curating this paper of yours for FlyBase: FBrf0073546 == Kernan et al., 1994, Neuron 12(6): 1195--1206 in which you list many larval touch insensitive lines in Table 1 (page 1198). Some of these you have assigned to complementation groups. My question is about the others. Have you assigned any of the remaining lines (e.g. 375, 70, 376 ...) to known complementation groups yet? If so, could you please provide me with the relevant gene symbols. If not, then we shall have to give these an arbitrary gene symbol, eg lbh1, lbh2 etc, (for larval behaviour gene 1, 2 etc) so I can capture data concerning the larval, adult and mosaic phenotypes. Many thanks for your help, Eleanor Whitfield FlyBase Subject: Re: Help FlyBase - larval touch insensitive lines Re: larval touch insensitives Hello Eleanor Sorry for the delay in replying to your inquiry - I have been away for 3 weeks teaching a summer course and am only now catching up on email. To answer your question about the lines mentioned in our Neuron paper - no more information, complementation or otherwise, is available than is presented in that table. (We have been concentrating on the unc and uncl -type mutations, for which we can define an electrophysiological phenotype.) If you wish to assign symbols to the lines (remembering that these are mutagenized chromosomes, rather than identified loci), how about til(touch-insensitive-larva)E, F, G...., for consistency with those that we have already named? tilA, by the way, has been lost. I appreciate all your efforts in curating the database - keep up the good work.... Maurice Kernan Subject: Re: Help FlyBase - larval touch insensitive lines Hi Maurice, >If you wish to assign symbols to the lines, how about >til(touch-insensitive-larva)E, F, G...., for consistency with those that we >have already named? that is fine, so this is the designation I shall be giving each line: line 375 tilE line 70 tilF line 376 tilG line 75 tilH line 411 tilI line 366 tilJ line 147 tilK line 350 tilL line 310 tilM line 301 tilN line 317 tilO line 78 tilP each shall have an allele number 1 so the larval, adult and mosaic phenotypes can be captured. > I appreciate all your efforts in curating the database - >keep up the good work.... Thank you very much. I shall curate this correspondance as a personal communication from you. Look forward to hearing from you soon, Eleanor Whitfield