Subject: Help FlyBase - Nrx map
Hi Troy,
I am a curator of FlyBase and at present I am working on your paper:
Baumgartner et al, 1996, Cell 87: 1059--1068
in which you list numerous complementation groups located in the Nrx region
(Fig 4A, pg 1063).
Do you know if these complementation groups are those already present in
FlyBase (see attached file) or have you isolated mutations of new
complementation groups?
If these complementation groups are new I shall have to rename them
continuing the 68F and 69A series:
68Fa - 68Fg
68Fb - 68Fh
.
69Aa - 69Ah
69Ab - 69Ai
The answer to this question is important as you state that l(3)68Ff is
allelic to Nrx, I do not want to incorrectly merge these two genes if your
68Ff is not the same complementation group as the 68Ff listed in FlyBase.
If you have any problems with this query please let me know as soon as
possible.
Regards,
Eleanor Whitfield
FlyBase
Attachment:
Translation of star codes:
\*a gene symbol
\*c cytological location
\*i synonym
\*A allele symbol
Genes present in FlyBase:
\*a l(3)68Fa
\*c 68E3--68F6
\*i l(3)F11
\*i l(3)V4-5
\*i l(3)rsg18
\*i rsg18: rose-gespleten region interval 18
\*A l(3)68Fa1
\*i l(3)FII
\*A l(3)68Fa2
\*i l(3)V4-1
\*A l(3)68Fa3
\*i l(3)V4-5ts
\*A l(3)68Fa4
\*i l(3)VII-5
\*a l(3)68Fb
\*c 68E3--68F6
\*i l(3)rsg19
\*i rsg19: rose-gespleten region interval 19
\*A l(3)68Fb1
\*i l(3)49
\*a l(3)68Fc
\*c 68E3--68F6
\*i l(3)rsg20
\*i rsg20
\*i l(3)68Fc: rose-gespleten region interval 20
\*A l(3)68Fc1
\*i l(3)BIP
\*i l(3)BIP
\*A l(3)68Fc2
\*i l(3)igh
\*a l(3)68Fd
\*c 68E3--68F6
\*i l(3)rsg21
\*i rsg21: rose-gespleten region interval 21
\*A l(3)68Fd1
\*i l(3)1B
\*a l(3)68Fe
\*c 68F3--68F6
\*i l(3)rsg22
\*i rsg22: rose-gespleten region interval 22
\*A l(3)68Fe1
\*i l(3)69
\*A l(3)68Fe2
\*i l(3)112.226
\*A l(3)68Fe3
\*i l(3)113
\*a l(3)68Ff
\*c 68F3--68F6
\*i l(3)rsg23
\*i rsg23: rose-gespleten region interval 23
\*A l(3)68Ff1
\*i l(3)21-2
\*A l(3)68Ff2
\*i l(3)112.139
\*A l(3)68Ff3
\*i l(3)112.143
\*A l(3)68Ff4
\*i l(3)112.186
\*A l(3)68Ff5
\*i l(3)112.25
\*A l(3)68Ff6
\*i l(3)DII
\*a l(3)69Aa
\*c 68F3--69A1
\*i l(3)rsg24
\*i rsg24: rose-gespleten region interval 24
\*A l(3)69Aa1
\*i l(3)145
\*a l(3)69Ab
\*c 68F3--69A1
\*i l(3)rsg25
\*i rsg25: rose-gespleten region interval 25
\*A l(3)69Ab1
\*i l(3)58-2
>
Hi Eleanor,
Troy passed me your email. None of the mutations present in Flybase were
available to us, even after repeatedly trying to obtain them. If you know
where we can find them, I'll be glad to do the complementation tests to
help clarify which one is which. We named them according to their
cytological location and the deficiency they fail to complement. Based on
our data we are almost sure that these new alleles are alleles of
previously isolated mutants described by Hoogwerf et al., as we found as
many complementation groups as they did within the respective areas. Since
these previous mutations are not available anymore (to our knowledge) we
did not want to name these genes differently. However, I understand that
we have not shown that 68Fa in Flybase is nrx IV, and therefore that we
should have chosen another name. Next time I will send you guys a paper
before publication so there is no screw-up in the nomenclature. Is this
sufficient information?
Hugo
Hugo Bellen, D.V.M., Ph.D.
Howard Hughes Medical Institute
Baylor College of Medicine
One Baylor Plaza - HHMI - Room T 630
Houston, TX 77030