Subject: transposon vector sequence
Dear Flybase,
A computer "restriction digest" of vector pP{DM23} shows it has 2 XbaI
sites, although the annotations show that XbaI is a unique site in the
polylinker (also stated in Mismer and Rubin 1987).  In my experience, there
is only one XbaI site in the polylinker (I have used it for cloning).  The
second "computer" XbaI site lies in the rosy sequence, and this is also in
the Genbank rosy gene sequence.
--Denise Clark
Department of Biology
University of New Brunswick
Fredericton, NB
Canada, E3B 6E1