John, >Is shof (shut off) = esg ? Michael Yes. Gary Hime says '...I am now convinced that shof is a specific allele of esg. Most of the P alleles cluster just 5' of the esg message, in the region that Shigeo Hayashi has found a testis specific esg enhancer. Also esg is expressed in precisely the cell types that I see affected.......the ammunition P was esgP2' (P2 is the name that Hayashi et al. 1993 Dev 118:105-115 gave to an insertion from Bier et al. 1989 Genes Dev. 3 1273-1287) John