Stanewsky, R., Jamison, C.F., Plautz, J.D., Kay, S.A., Hall, J.C. (1997). Multiple circadian-regulated elements contribute to cycling period gene expression in Drosophila. EMBO J. 16(16): 5006--5018.
FlyBase ID
FBrf0096339
Publication Type
Research paper
Abstract
A new regulatory element necessary for the correct temporal expression of the period (per) gene was identified by monitoring real-time per expression in living individual flies carrying two different period-luciferase transgenes. luciferase RNA driven from only the per promoter was not sufficient to replicate the normal pattern of per RNA cycling; however, a per-luc fusion RNA driven from a transgene containing additional per sequences cycled identically to endogenous per. The results indicate the existence of at least two circadian-regulated elements--one within the promoter and one within the transcribed portion of the per gene. Phase and amplitude analysis of both per-luc transgenes revealed that normal per expression requires the regulation of these elements at distinct phases and suggests a mechanism by which biological clocks sustain high-amplitude feedback oscillations.
