Subject: Helping FlyBase
Dear Troy,
I am curating your Neuron paper for FlyBase:
Temperature sensitive paralytic mutations demonstrate that synaptic
exocytosis requires SNARE complex assembly and disassembly
Neuron 21: 401-413
and I have a question for you. Figure 1 shows the effect of rescuing
'ts3-69' with a wild type copy of the syx1A gene. However you do not
describe or give a reference for the wild type syx1A transgene.
Is it this one?
\*A syx1A+t6
\*z FBal0056009
\*x FBrf0091165 == Schulze and Bellen, 1996, Genetics 144(4): 1713--1724
\*i unnamed
\*o in vitro construct | regulatory fusion
\*I P{syx+6}
\*k Mode of assay: In transgenic Drosophila
\*Q Rescues: syx1A06737
\*Q Rescues: syx1AΔ229
\*Q Lethality of syx1A06737 and syx1AΔ229 is rescued.
\*s 6.0kb NotI fragment encompassing the syx1A open reading frame and
\*s up and down sequences.
Many thanks for your help,
best wishes,
Rachel.
Subject: Re: Helping FlyBase
Dear Rachel,
The syntaxin rescue construct is actually P{syx+13}, but it encompasses
the same syx1A reading frame as Psyx6.
Thanks, Troy