Subject: FlyBase query
Dear Gunter,
in October you wrote to FlyBase, pointing out that the FlyBase gene
with the ID number FBgn0008635 codes for beta-COP (we had it wrongly as
beta-prime-COP) and that you had cloned the 'real' beta-prime-COP.
Here is a copy of the e-mail you sent:
> Subject: FBgn0008635
> Date: Thu, 15 Oct 1998  15:24:32  +0200
> Content-Length: 1067
>
> Dear colleagues,
>
> The gene 'beta-prime coatomer protein' with the FlyBase ID number FBgn0008635
> indeed codes for 'beta-COP' according to its homology to beta-COP proteins from
> a variety of species and according to the orig. paper from Ripoche et al. 1994.
> It should therefore be renamed.
>
> I have recently cloned the cDNAs for the 'real' Drosophila beta-prime subunit
> and the alpha COP subunit:
>
> beta-prime COP:
>
> gene: Drosophila m. coatomer beta-prime subunit; beta'-COP; beta-prime COP
> cytological localization: 34B5-34B9
> EMBL-Acc-No: AJ006523 (cDNA)
> AJ006524 (genomic)
>
> alpha COP:
>
> gene: Drosophila m. mRNA for coatomer alpha subunit; alpha-COP
> cytological localization: 62A10-62B5
> EMBL-Acc-No: AJ011114
>
> Please feel free to contact me if you have any questions or need any additional
> information.
>
> With best wishes,
>
> Gunter Merdes
>
> ZMBH
> University of Heidelberg
> Im Neuenheimer Feld 282
> 69120 Heidelberg
> Germany
>
>
When I was looking at our files and your e-mail, I realised that in
your e-mail you have given cytological information for both
beta-prime-COP and alpha-COP that we do not have in our files and is
not in the DNA accession records for these genes. The reason I am
writing to you is that I would like to include this information,
specifically:
beta-prime COP: cytological localization: 34B5-34B9
alpha COP: cytological localization: 62A10-62B5
as a personal communication from you to FlyBase, as it is always very
useful to have cytological information about genes. Am I correct in
thinking that you obtained the cytology by in situ hybridisation
(rather than deficiency mapping) - as we like to include the method by
which the cytology was determined, if possible.
I look forward to hearing from you,
Gillian
\--------------------------------------------------------------
Gillian Millburn.
FlyBase (Cambridge),
\--------------------------------------------------------------
>
Subject: COP
Dear Gillian,
the cytology was indeed obtained by in situ hybridisation and verified by
comparison of my sequences with the already sequenced P1 clones from BDGP.
Gunter
Gunter Merdes
ZMBH
University of Heidelberg
Im Neuenheimer Feld 282
69120 Heidelberg
Germany