With regard to the Ubi-GFP insertions, this is what I know: The insertion on 3R does indeed map to polytene internal 83. This is a jump that I isolated myself from one of Ilan Davis' original lines. I told the lucky technician who mapped the jump not to bother defining the map position beyond a numbered division. I'm not so sure about the insertion(s) on 2L. I isolated 1 jump myself at 36, and mapped two of Ilan's original lines to 33 and 38. I made recombinants of these insertions with FRT40 in various combinations, and retained two lines, each with two insertions. One line was 33+38 and the other 36+38. And at one time I also had lines with only 1 Ubi-GFP insertion on the FRT40 chromosome. I think I sent either 33+38 FRT40 or 36+38 FRT40 to David Bilder, but I don't have any record of this. If he doesn't know either, then the information is lost. I'll happily send you a line which has insertions in known positions! I also have a line with an insertion on the X recombined with FRT18neo, which is a natural partner to these other lines. I don't know where the insertion in this maps though... I hope this is of help. Best regards, David Strutt ----------------------------------------------------------------------------- Dr. David Strutt Developmental Genetics Programme University of Sheffield Firth Court Western Bank Sheffield S10 2TN England