The following data was provided by Stefan Knirr Mount Sinai Medical Center New York, NY 10029 In the insc allele insc-delta 13 the P-element is inserted after the 13th base pair of the transcriptional start site. The sequence in accession U73489 starts with base 1 of the transcription. ____________________________________________________________________ Subject: Where does the P8 insertion map in insc? Dear Dr. Knirr, I am a molecular curator for FlyBase and I am working on the annotation of the fly genome. I was hoping you could help with me a question about insc. In your 1997 Mechanisms of Development paper, it says that the inscP8 insertion maps 13 bases downstream of the transcription start of insc. My problem is that I don't know where the transcription start is. Does the cDNA sequence in accession U73489 start with base 1 of the transcript? The start of the sequence is as follows: ATCAGAAGTC TGAGTCGAAG Is the site of insertion before or after the 13th base (an A) or somewhere else? If you can clarify this for me, I'll include it in the annotation along with the mapping of inscdelta13 and both will be referenced to your paper. Thanks for your help, Beverley Matthews
