Personal communication to FlyBase, 22 July 1999, from David Micklem,
Dept. of Biochemistry, Stanford University, Stanford, CA, USA.
Corrections and additions to compiled sequence of pPacPL.
LOCUS PPAC-PL.GE 6429 BP SS-DNA CIRCULAR SYN 07-JAN-1993
DEFINITION pPacPL Vector for transient expression in Drosophila cell cultures
ACCESSION -
KEYWORDS -
SOURCE Synthetic construct DNA.
ORGANISM synthetic construct artificial sequence.
FEATURES Location/Qualifiers
frag 2612..2986
/note="Confirmed by sequencing"
frag 4905..5316
/note="Confirmed by sequencing"
source 1..2400
/note="/segment=""pUC18(S) (GB/L08752 287..2686)"""
source 2401..2628
/note="/segment=""pUC18(N) (GB/L08752 1..229)"""
source 2629..2634
/note="/segment=""J EcoRI"""
source 2635..2662
/note="/segment=""Act5C_5' (R1,u)0.2 - now sequenced, and
is only 27bp, not 200bp"""
source 2663..4595
/note="/segment=""Act5C_P2.45 (GB/X15730 1..????)"" with
minor changes from DRM sequencing"
source 4596..4599
/note="/segment=""J filled in SpeI site"" (not in original
flybase sequence)"
source 4600..5121
/note="/segment=""Act5C_P2.45 (GB/X15730
????..2449)"" "
source 5122..5209
/note="/segment=""Act5C+0.088 (GB/X06382 205..292)"""
source 5210..5274
/note="/segment=""J pacPL.mcs now sequenced"
source 5275..6386
/note="=""Act5C+1.1(S) (GB/X06384 7..1118)"" "
source 6387..6429
/note="/segment=""J 001193"""
unsure 5164..5164
/note="C or G Both variants are present in the Krasnow
lab. Avoid using this region for sequencing primers."
unsure 3047..3047
/note="This A probably present(not in old flybase sequence
but my sequence quality marginal)"
variation 2892..2892
/note="Additional G (vs old flybase sequence)"
variation 2853..2854
/note="Additional CA (vs old flybase sequence)"
variation 2842..2842
/note="G (vs C in old flybase sequence)"
variation 2840..2840
/note="Additional A (vs old flybase sequence)"
variation 2664..2664
/note="Additional A (vs old flybase sequence)"
variation 2620..2620
/note="Only single C (vs CC in old flybase sequence)"
BASE COUNT 1814 A 1494 C 1442 G 1679 T 0 OTHER
ORIGIN -
1 GGCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA
61 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA
121 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT
181 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC
241 TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG
301 GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT
361 GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG
421 CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT
481 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA
541 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT
601 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT
661 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG
721 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA
781 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG
841 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT
901 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG
961 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG
1021 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA
1081 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC
1141 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC
1201 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC
1261 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG
1321 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC
1381 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC
1441 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT
1501 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC
1561 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA
1621 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC
1681 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT
1741 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG
1801 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC
1861 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT
1921 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA
1981 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT
2041 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG
2101 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA
2161 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA
2221 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT
2281 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
2341 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
2401 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
2461 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT
2521 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT
2581 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC ATGATTACGA ATTCTATATT
2641 CTAAAAACAC AAATGATACT TCTAAAAAAA AATCATGAAT GGCATCAACT CTGAATCAAA
2701 TCTTTGCAGA TGCACCTACT TCTCATTTCC ACTGTCACAT CATTTTTCCA GATCTCGCTG
2761 CCTGTTATGT GGCCCACAAA CCAAGACACG TTTTATGGCC ATTAAAGCTG GCTGATCGTC
2821 GCCAAACACC AAATACATAA TGAATATGTA CACATTCGAG AAAGAAGCGA TCAAAGAAGC
2881 GTCTTCGGGC GGAGTAGGAG AATGCGGAGG AGAAGGAGAA CGAGCTGATC TAGTATCTCT
2941 CCACAATCCA ATGCCAACTG ACCAACTGGC CATATTCGGA GCAATTTGAA GCCAATTTCC
3001 ATCGCCTGGC GATCGCTCCA TTCTTGGCTA TATGTTTTTC ACCGTTACCC GGGGCCATTT
3061 TCAAAGACTC GTCGGCAAGA TAAGATTGTG TCACTCGCTG TCTCTCTTCA TTTGTCGAAG
3121 AATGCTGAGG AATTTCGCGA TGACGTCGGC GAGTATTTTG AAGAATGAGA ATAATTTGTA
3181 TTTATACGAA AATCAGTTAG TGGAATTTTC TACAAAAACA TGTTATCTAT AGATAATTTT
3241 GTTGCAAAAT ATGTTGACTA TGACAAAGAT TGTATGTATA TACCTTTAAT GTATTCTCAT
3301 TTTCTTATGT ATTTATAATG GCAATGATGA TACTGATGAT ATTTTAAGAT GATGCCAGAC
3361 CACAGGCTGA TTTCTGCGTC TTTTGCCGAA CGCAGTGCAT GTGCGGTTGT TGTTTTTTGG
3421 AATAGTTTCA ATTTTCGGAC TGTCCGCTTT GATTTCAGTT TCTTGGCTTA TTCAAAAAGC
3481 AAAGTAAAGC CAAAAAAGCG AGATGGCAAT ACCAAATGCG GCAAAACGGT AGTGGAAGGA
3541 AAGGGGTGCG GGGCAGCGGA AGGAAGGGTG GGGCGGGGCG TGGCGGGGTC TGTGGCTGGG
3601 CGCGACGTCA CCGACGTTGG AGCCACTCCT TTGACCATGT GTGCGTGTGT GTATTATTCG
3661 TGTCTCGCCA CTCGCCGGTT GTTTTTTTCT TTTTATCTCG CTCTCTCTAG CGCCATCTCG
3721 TACGCATGCT CAACGCACCG CATGTTGCCG TGTCCTTTAT GCGTCATTTT GGCTCGAAAT
3781 AGGCAATTAT TTAAACAAAG ATTAGTCAAC GAAAACGCTA AAATAAATAA GTCTACAATA
3841 TGGTTACTTA TTGCCATGTG TGTGCAGCCA ACGATAGCAA CAAAAGCAAC AACACAGTGG
3901 CTTTCCCTCT TTCACTTTTT GTTTGCAAGC GCGTGCGAGC AAGACGGCAC GACCGGCAAA
3961 CGCAATTACG CTGACAAAGA GCAGACGAAG TTTTGGCCGA AAAACATCAA GGCGCCTGAT
4021 ACGAATGCAT TTGCAATAAC AATTGCGATA TTTAATATTG TTTATGAAGC TGTTTGACTT
4081 CAAAACACAC AAAAAAAAAA ATAAAACAAA TTATTTGAAA GAGAATTAGG AATCGGACAG
4141 CTTATCGTTA CGGGCTAACA GCACACCGAG ACGAAATAGC TTACCTGACG TCACAGCCTC
4201 TGGAAGAACT GCCGCCAAGC AGACGATGCA GAGGACGACA CATAGAGTAG CGGAGTAGGC
4261 CAGCGTAGTA CGCATGTGCT TGTGTGTGAG GCGTCTCTCT CTTCGTCTCC TGTTTGCGCA
4321 AACGCATAGA CTGCACTGAG AAAATCGATT ACCTATTTTT TATGAATGAA TATTTGCACT
4381 ATTACTATTC AAAACTATTA AGATAGCAAT CACATTCAAT AGCCAAATAC TATACCACCT
4441 GAGCGATGCA ACGAAATGAT CAATTTGAGC AAAAATGCTG CATATTTAGG ACGGCATCAT
4501 TATAGAAATG CTTCTTGCTG TGTACTTTTC TCTCGTCTGG CAGCTGTTTC GCCGTTATTG
4561 TTAAAACCGG CTTAAGTTAG GTGTGTTTTC TACGACTAGC TAGTGATGCC CCTACTAGAA
4621 GATGTGTGTT GCACAAATGT CCCTGAATAA CCAATTTGAA GTGCAGATAG CAGTAAACGT
4681 AAGCTAATAT GAATATTATT TAACTGTAAT GTTTTAATAT CGCTGGACAT TACTAATAAA
4741 CCCACTATAA ACACATGTAC ATATGTATGT TTTGGCATAC AATGAGTAGT TGGGGAAAAA
4801 ATGTGTAAAA GCACCGTGAC CATCACAGCA TAAAGATAAC CAGCTGAAGT ATCGAATATG
4861 AGTAACCCCC AAATTGAATC ACATGCCGCA ACTGATAGGA CCCATGGAAG TACACTCTTC
4921 ATGGCGATAT ACAAGACACA CACAAGCACG AACACCCAGT TGCGGAGGAA ATTCTCCGTA
4981 AATGAAAACC CAATCGGCGA ACAATTCATA CCCATATATG GTAAAAGTTT TGAACGCGAC
5041 TTGAGAGCGG AGAGCATTGC GGCTGATAAG GTTTTAGCGC TAAGCGGGCT TTATAAAACG
5101 GGCTGCGGGA CCAGTTTTCA TATCACTACC GTTTGAGTTC TTGTGCTGTG TGGATACTCC
5161 TCCCGACACA AAGCCGCTCC ATCAGCCAGC AGTCGTCTAA TCCAGAGACC CCGGATCCGA
5221 TATCACTAGT TCTAGAGGTA CCGAGCTCGC GGCCGCGTTA ACCGATCCGT CGACCATGAA
5281 GATCAAGATC ATTGCCCCGC CAGAGCGCAA GTACTCTGTC TGGATCGGTG GCTCCATCCT
5341 GGCTTCGCTG TCCACCTTCC AGCAGATGTG GATCTCCAAG CAGGAGTACG ACGAGTCCGG
5401 CCCCTCCATT GTGCACCGCA AGTGCTTCTA AGAAGGATCG CTTGTCTGGG CAAGAGGATC
5461 AGGATCGGGA TGGTCTTGAT TCTGCTGGAG GAGGAGGAGG AGAAGTCGAG GAAGCAGCAG
5521 CGAAAGTGCA AGTGCGAGTG GTGGAAGTTT GGAGTGCAGC ACAACAAAAT CAACAACAAC
5581 ACCAACTACA AGATGAAAAG AGCGGAACCA CCTGCACACC ATCATCACTA TCATCATCGT
5641 TTTGGGCGCA TGTTGTGTGG TTCCAGCGTA TTAATATAAT TAATTTATTC CACATGAGAT
5701 ATGATATGAT ATACTATGTA TTTTTTGTTT TTTTTTTATT TGTAAACCTT TAATATAACA
5761 AGAACTACAA AAAAGTGAAA ATGAGCGAAA ATGCATATTC TGCCATTCCA CACACACACC
5821 AACAACACCC AACACACGCA CACCCACAAG CTTACACACA CACACATTCG CGGCATGACA
5881 AGGACATCAA GATAAAGAAG AACTTAAAGA AGATATTTCC CAAAGCGCAA AAAGAACACA
5941 CACACATTGC AAAACACAAA CAACACACTA GCGTTTTGTA CAATTCGTCA GCAACCTTAT
6001 GTATTATTTT TTAATTATGA TGTAATTATA AACAAAGTGA AACAAAAATA TGAAAACAAA
6061 AAGGAAAATC AAATCTGTCT TCTCTTTCTC CCGCTCTCCT CGCTCTCTGC TGCTAACCTC
6121 GCCCTCTCCT CTCTCATCTT TTTGTCTGTC TCTCTTCACA TTTTTGCCGG CCGGCAAAAT
6181 AATAACCCAC ACACACTCAC ACTTGGCTGC AGTTTCGCGT GCGATATTCA CACACATTCA
6241 AGCATACATA CATATGTATT TTTTTTTTAT TTGTACACTT TTCTAATTGC ATGCGTATCG
6301 ATTGATAAGT TTACGCTGAA AATGTTAATT AAAATGTGAA AATGCAACTG AAAAACTGAT
6361 GAAATGAAAC AACAACAAGC GAACAAGTCG ACTCTAGAGG ATCCCCGGGT ACCGAGCTCG
6421 AATTAATTC
