Personal communication to FlyBase from Ulrich Nauber, received 08/30/99.
Concerning knirps constructs mentioned in Figure 2 of Lunde et al., 1998, 
Development  125:4145 .
In the legend of Figure 2 TWO constructs are mentioned:
One is a genomic fragment tested for rescue, the other one is a kni-lacZ
reporter construct. Both constructs and the resulting fly stocks were made
in the lab of Herbert Jaeckle.
The rescue construct consists of a 9.5 kb Sal I / Hind III fragment that
contains about 5.5 kb of upstream sequences, the complete kni transcription
unit (3 kb) and about 1 kb of downstream sequences. This genomic fragment
was inserted into the Carnegie 20 vector and transformed into flies. A map
of the construct is published in Pankratz et al., 1992 (Science 255,
986-989). The resulting fly stock is named P(ry+; kni-42) and is available
from Herbert Jaeckle.
The kni-lacZ reporter construct is a 4.4 kb Bam HI / Nru I upstream
fragment of kni fused to the pCaSpeR AUG beta-gal. It is described in
detail (with map) in Pankratz et al., 1989 (Nature 341, 337-340) and in
Pankratz et al., 1992 (Science 255, 986-989). The fly stock is named P(w+;
4.4knilacZ/2-69) and is available from Herbert Jaeckle.
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Dr. Ulrich Nauber
Max-Planck-Institut fuer biophysikalische Chemie
Abt. Molekulare Entwicklungsbiologie (Abt. 170)
Am Fassberg 11
D-37077 Goettingen
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