Subject: RE: FlyBase Query
The B118 deletion I referred to in my conference abstract is associated with
the T(1;Y)B118 translocation. Kevin Mitchell, formerly of Corey Goodman's
lab, had been doing some physical mapping in the region of the NetA and NetB
genes, which are located in the 12E-F region. As part of his studies, he had
mapped the approximate breakpoints of some X;Y translocations with previously
reported breakpoints in the 12E-13A region. He indicated to me that
T(1;Y)B118 had a breakpoint on the X chromosome between NetB and rut. The two
genes I study are Agr and Agr2, both of which are located in 12F between NetB
and rut. I was interested in positioning the breakpoint of this translocation
in relation to Agr and Agr2. Many males carrying the translocation are both
viable and fertile (actually about 50-60% seem to die during the larval
stage), so as a first experiment, I simply extracted genomic DNA from these
males and did a Southern, using labeled Agr and Agr2 cDNAs. To my surprise,
the 3' half of Agr and all of Agr2 appears to be missing. I have since
repeated this experiment several times, with identical results. I have tried
to determine the extent of this deletion: One breakpoint occurs within the
Agr gene; the other appears to be 20-25 kb away. I don't believe any of the
rut gene is deleted, which is consistent with the literature. I also don't
believe that any of the NetB coding region is deleted. I haven't precisely
mapped the location of NetB or rut in relation to Agr or Agr2, so this
information is really an educated guess. I hope to gather some more precise
information once I've re-established my lab in Texas. If you need any
additional information, please let me know. . I hope this information clarifies things for you.
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Dear Dr Kovalick,
I am curating your abstract for FlyBase:
Kovalick and Ray, Society for developmental Biology, 58 1999
(as published in Developmental Biology 210(1) 209 1999)
You mention a chromosomal aberration B118. We do have in our records
a B118 translocation, T(1;Y)B118:
\*a T(1;Y)B118
\*z FBab0006664
\*B 12F1;h1-h17
\*c All limits from polytene analysis (citation unavailable)
\*G l(1)dd4 << bk1 << NetA << rut << bk2
\*w Merriam and colleagues.
\*o X ray
\*O Dp(1;Y)BSYy+
\*p male viable
\*x FBrf0066905 == Lindsley and Zimm, 1992, book
\*x FBrf0102049 == Merriam et al., 1998.4.24, personal communication
However your abstract seems to suggest that your 'B118' is new and
distinct from this one. Is your 'B118' a straightforward deficiency?
If so, is it about to be published anywhere? If not please could
you send me a bit more information (discoverer, mutagen, breakpoints
...). This information is very useful to us as it impinges on the map
data for the 'Agr2' gene.