Subject: RE: question from FlyBase
         Reply to:   RE: question from FlyBase
Dear Beverley,
Well, I actually was able to go back to Robert Clifford's thesis, which has more 
data than what was published. In his thesis, he actually says that the basepair 
change affects bp 2469 and amino acid Cys 655 to Ser. According to your email 
this would make more sense. I have no idea why in the Table 2 of his paper Cys 
662 is listed. The only thing I can imagine is that a typo was "corrected" along 
the way, making matters worse. Anyway, it should be 655 from all I can tell. And 
by the way, we also sent in another correction to the sequence in GenBank in 
recent years. But you probably know all that.
Best wishes.
Yours, Trudi. 
>Date: Mon, 20 Sep 1999  11:40:47  -0400 (EDT)
>Subject: question from FlyBase
>Dear Dr. Schupbach,
>
>I am a curator for FlyBase and am working on the annotation of the
>Drosophila genome. I was marking the sites of mutations of Egfr and ran
>across an ambiguity. Egfrf37 (referred to as Egfr4A in your '94
>Clifford and Schupbach Genetics paper ( 137:531-550 ) is marked as having
>a change from TGC to AGC at genomic position 2469 that leads to amino
>acid change Cys 662 to Ser. The problem is that genomic position 2469
>does not correspond to amino acid 662. The change may have been at 2469
>(amino acid 655) or it may have been at 2490 (amino acid 662). The same
>TGC codon occurs in both places. I'm not sure whether the nucleotide
>position or the amino acid position was the typo.
>I know it can be very difficult to track down details like this years
>after the experiments were done but if you have any way of checking or
>can tell me who to ask, I'd love to get the correct information for
>the annotation.
>Thanks for your help,
>Sincerley,
>
>Beverley Matthews
>FlyBase-Harvard