Subject: flybase
Dear Chihiro,
..
By the way, there is a mistake in our Table 2. The cytological interval
for tumbleweed is 49C-50D, and the interval for shrub is 44F-45E. The two
was reversed in the Table. it would be nice if you could change that when
you put the information into Flybase.
Thanks.
With best wishes.
Fen-Biao
Jan Lab
\--
Subject: FlyBase Query
Dear Dr Jan,
I am currently curating your paper for FlyBase:
Gao et al, 1999 Genes Dev. 13(19) 2549-2561.
I have a few questions I was hoping you could answer for me.
1. yew allele
In Table 2 you mention an allele of a your new gene yew. Do you have a
name for this allele? (e.g. yew1)
2. short symbols, limber, ponderosa, cypress, saguaro, (yew)
Also in table 2 you mention a number of new genes. Have you decided on short symbols for these genes? Here are a number of suggestions that haven't already been used, though if you want to use another symbol that hasn't been taken then that is, of course, no problem. I assume that 'ye
limber
(lim is already taken)
lmb
lmr
lbr
lmbr
ponderosa
(pon is already taken)
pond
pnd
cypress
(although cyp is not taken, Cyp1, Cyp4c3, and other similar
symbols are already in use)
cps
cypr
cyps
saguaro
(sag is already taken)
sago
sgu
sgo
sgr
3. UAS-GFP
You use a UAS-GFP construct throughout your paper, there are a number
in our records, could you tell me if the one you used in this paper is
one of those below ( I have listed those that seem to be the most
likely ones). If it is not, or you are not sure, could you tell me
where you got the construct from, and any other papers you know that
this construct has been used in before.
Avic\GFPm6.Scer\UAS
Brand, 1999, Methods Cell Biol. 58: 165--181
P{UAS-GFP.m6}
UAS-mGFP6
J. Haseloff
C.M. Davidson
A.H. Brand
Expression of GFP carrying amino acid substitutions (for
brighter fluorescence, higher solubility and improved
maturation and spectral properties) is governed by UAS
regulatory sequences.
Avic\GFPS65T.Scer\UAS
Timmons et al., 1997, Dev. Genet. 20(4): 338--347
GFP is expressed under the control of UAS regulatory sequences
Avic\GFPS65T.I167T.Scer\UAS
Brand, 1999, Methods Cell Biol. 58: 165--181
P{UAS-GFP.S65T.I167T}
UAS-GFP S65T/I167T
C.M. Davidson
A.H. Brand
Expression of GFP carrying two amino acid substitutions for
brighter fluorescence is governed by UAS regulatory sequences.
Amino acid replacement: S65T.
Amino acid replacement: I167T.
Avic\GFPScer\UAS.cGa
GFPUAS.cGa
Santel et al., 1998, J. Cell Sci. 111(22): 3299--3309
Saccharomyces cerevisiae UAS construct a of Gonzalez-Gaitan
P{UAS-GFP.G}
M. Gonzalez-Gaitan
UAS regulatory sequences drive expression of Avic\GFP
Avic\GFPScer\UAS.cYa
Yeh et al., 1995, Proc. Natl. Acad. Sci. USA 92(15): 7036--7040
Saccharomyces cerevisiae UAS construct a of Yeh
P{UAS-GFP.Y}
KpnI-EcoRI cDNA fragment of GFP is expressed under the
transcriptional control of five GAL4 UAS sites.
Thank you for answering these questions.
Best Wishes,
Chihiro