Subject: Re: Correction of info at
http://fruitfly.berkeley.edu/sequence-archive.html
Rachel-
Thanks for responding. Z(210), which we call zetalin, and
Z(400/600)are both Z-band proteins in Drosophila flight muscle. THE LOCI FOR
THE GENES ENCODING THEM HAS NOT BEEN DETERMINED, AND NEITHER ONE OF THEM IS
REPRESENTED BY GenBank accession \#U51473. The cDNA that localizes to 52D and
has been partially sequenced (U51473) codes for a previously unidentified
225kD protein that is associated with the A-band of indirect flight muscle.
It is unrelated to any protein, such as Z(210) or Z(400/600) mentioned in
FBrf0050188 or FBrf0054488.
The problem is that the clone we isolated and sequenced we thought
coded for zetalin, but we were mistaken. The title of the unfortunate
abstract <up>1997, Molec. Biol. Cell(FBrf0100232)</up> should have read '225kD
protein, an A-band associated component in Drosophila flight muscle, has
repeating immunoglobulin C2-like domains' instead of 'Zetalin, a major
Z-band component....'. We learned of our mistake after making POLYCLONAL
antibodies to the protein expressed by our cDNA and found that THEY labelled
neither the Z-band of indirect flight muscle nor the zetalin doublet on
immunoblots of fly flight muscle separated be SDS-PAGE. THEY labelled the
A-band region of myofibrils, and on immunoblots THEY bound to a single
protein band, 225kD, that migrated extremely closely to the lower molecular
weight component of zetalin.
The point is that Z(210),aka zetalin,(FBrf0050188), and FBrf0054488
should NOT be linked to (U51473)/52D. The link is between a novel 225kD
A-band associated protein and (U51473/52D).
Judith D. Saide, Ph.D.
Dept. of Physiology, L-713
Boston Univ. Sch. of Med.
80 E. Concord St.
Boston, MA 02118