Subject: Re: FlyBase Query (cy269)
Dera Chihiro,
The 44 copies were oligomerised in vitro. They originate from an
unpublished RNA sequence that binds to rhodamine on a column (but not in
vivo in flies) that was in vitro selected (SELEX). It is 44 copies of
approx. 100bp sequence which is non-coding and originally made as DNA
oligonucleotides.
I hope this answers your questions.
with best wishes
ilan
>Dear Dr Davis,
>
>I am currently curating your paper for FlyBase:
>
>Wilkie et al. 1999 Current Biology 9(21) 1263-1266.
>
>I have a question I was hoping you could answer for me.
>
>hb-X44
>
>In fig 4. and the supplementary material, you mention a construct X44,
>that consists of three bcd-binding sites from a 0.75kb fragment of
>the hb promoter driving the expression of 44 tandem non-coding
>repeats and lacking polyadenylation and transcription termination
>sequences.
>
>Could you tell me where the 44 tandem non-coding repeats came from?
>Are they a known class of repeat (e.g. opa repeats)? Were the 44
>repeats found as a whole from genomic DNA, or were they oligomerised
>in vitro?
>
>Thanks
>
>Chihiro
>
>----------------------------------------------------------------------
>Chihiro Yamada.
>
>FlyBase (Cambridge),
>----------------------------------------------------------------------
____________________________________________
Dr Ilan Davis
Wellcome Institute for Cell Biology
ICMB, King's Buildings, Edinburgh University
Mayfield Road, Edinburgh EH9 3JR, Scotland
______________________________________________