A comparison of
a). the positions of predicted genes in the EDGP sequence and
b). restriction maps from the EDGP sequence
with the data provided by Juan (see below) indicates that
l(1)EC4 =  EG:165H7.3 .
FROM JUAN:
Francisco González, in his Ph.D. thesis (González, 1989),
started to characterize the region proximal from AS-C T1. He
obtained a lambda phage (lsc7R) extending for ca. 12 kb
proximally from the end of our walk in the AS-C (which is 9.5
kb proximally from the start of ase) and found that it
hybridized to several transcripts (1.0, 0.8 and 5.5 kb). The
5.5 kb transcript was present in embryos, larva, early pupa
and adults. It was lit up by each of the four fragments (Eco-
Bam4.1 kb (fragment I), Bam-Bam4.2 kb (fragment II), Bam-
Bam3.2 kb (fragment III), and Bam-Eco1.2 kb (fragment IV),
distally to proximally) that comprised the 12 kb insert of
lsc7R, suggesting a transcription unit that gives rise to a
transcript which is subsequently spliced. He also found in
southern blots the following modifications: Df(1)260.1
modifies frag. I. So prox. breakpoint of Df. at 9.5 to 13.5
kb to the left of start of ase. Df(1)scB57 modifies frag. II.
So prox. breakpoint of Df. at 13.5 to 17.7 kb to the left of
start of ase. l(1)EC4S1 modifies frag. I and l(1)EC4S211
modifies frag. II. So, we think that the transcript
corresponds to the gene l(1)EC4 found by Kalpana White, as
cited by Jimenez and Campos-Ortega (Jiménez and Campos-
Ortega, 1987). Whether  EG:165H7.3  or any of the more proximal
predicted genes up to svr correspond to l(1)EC4, I do not
know. You could probably find out by comparing the positions
of the predicted genes with the above data.......
The genetic analyses of Kalpana also placed
l(1)EC5 between l(1)EC4 and svr.
With regards to the data on l(1)EC4, the BamH1 site in
between the 4.0 Eco-Bam and the 4.2 Bam-Bam fragments (the
two fragments modified in the l(1)EC4S1 and l(1)EC4S211
alleles, respectively, which also contain the breakpoints for
Df(1)260.1 and Df(1)scB57, respectively again) is at position
30.771 of the cosmid 165H7 sequence, acces. n. AL009188.
Encarna dixit. It may help you in identifying one of the
predicted genes with l(1)EC4