Subject: Re: FlyBase Query (cy456)
Dear Chihiro,
I have indeed made the UAS-Antp construct and flies cited in Kurata et al.
For this I isolated an Antp cDNA of 2,2 kb as a NotI fragment from pNHTA
(Gibson et al,1990, Cell 62: 1087-1103) and inserted it in the pUAST vector
of Andrea Brand (Brand and Perrimon, 1993, Development  118:401-415 ) at the
same site, orientation selected by restriction digest. As far as I am aware
this is the first time this construct appears in a published paper.
Please let me know if you need further informations.
Best regards,
Bruno
Bruno Bello
Mammalian Development
National Institute for Medical Research
London Mill Hill NW7 1AA
Subject: FlyBase Query (cy456)
Dear Dr Bello,
I am currently curating a paper for FlyBase:
Kurata et al., 2000, Proc. Natl. Acad. Sci. 97(5): 2117--2122
The authors in this paper have cited you as the source for the UAS-Antp
strain of flies. I have a question about these flies was hoping you
could answer for me:
UAS-Antp
There are three UAS-Antp alleles in our files. Is the one you gave to
Kurata et al one of these? If it is not, or you are not sure, could
you provide me with some details about this construct. Did you make
this construct? What are the molcular details of this contruct? Are
there any other papers that you know of that this construct has been
used?
AntpScer\UAS.cCa
Roy et al., 1997, Curr. Biol. 7(4): 222--227
Saccharomyces cerevisiae UAS construct a of Carroll
provided by Sean Carroll.
in vitro construct | regulatory fusion
P{UAS-Antp}
AntpScer\UAS.cKa
Heuer et al., 1995, Development 121(11): 3861--3876
Li and McGinnis, 1999, Proc. Natl. Acad. Sci. USA 96(12): 6802--6807
Yao et al., 1999, Dev. Biol. 211(2): 268--276
Saccharomyces cerevisiae UAS construct a of Kalkbrenner
in vitro construct | regulatory fusion
P{UAS-Antp.K}
AntpScer\UAS.cMa
Azpiazu and Morata, 1998, Genes Dev. 12(2): 261--273
Casares and Mann, 1998, Nature 392(6677): 723--726
Ryoo and Mann, 1999, Genes Dev. 13(13): 1704--1716
Saccharomyces cerevisiae UAS construct a of Moreno
Gift from Ecuador Moreno.
in vitro construct | regulatory fusion
P{UAS-Antp.M}
Thanks for your time and best wishes,
Chihiro
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Chihiro Yamada.
FlyBase (Cambridge),
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