Subject: Helping FlyBase: ADRC-727C
Dear Sergei,
We are currently curating the abstracts for the upcoming 41st
(Pittsburgh) Annual Drosophila Research Conference, for FlyBase.
I am writing in connection with your abstract:
'From phenotype to gene function: A molecular screen for novel genes
required for the development of the peripheral nervous system.'
Your abstract suggests that Calreticulin and pot pourri are allelic,
but since we have somewhat different map locations for these geens in
FlyBase (potp: 85E and  Crc:86B--86C ) I wanted to be sure that is what
you meant before I merge the gene records.
Thank you for your help,
with best wishes,
Rachel.
Subject: Re: Helping FlyBase: ADRC-727C
Dear Rachel,
> Your abstract suggests that Calreticulin and pot pourri are allelic,
> but since we have somewhat different map locations for these geens in
> FlyBase (potp: 85E and  Crc:86B--86C ) I wanted to be sure that is what
> you meant before I merge the gene records.
Yes, this is exactly what I meant. I have isolated plasmid rescues from
the 1143/7 potp P element and found that their sequence is identical to
the published mRNA sequence of calreticulin. Plasmid rescues where mapped
to the same cytological location as the P insertion (85E).
In addition, we mapped the LD07621 calreticulin cDNA (BDGP) to 85E1.
Therefore, I concluded that the published mapping position for
calreticulin (86B-C) is incorrect.
Please, let me know if you have any further question. If it can be of help
for you, I would be glad to send you a copy of the manuscript on molecular
screen when we submit it to Genetics by the end of March.
Best wishes,
Sergei
Sergei N. Prokopenko
Program in Developmental Biology
Baylor College of Medicine
Rm. T634, Mail stop BCM235
One Baylor Plaza
Houston, TX 77030, USA
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