Gene or accession: CG6246
Gene annotation error
Gene CG6246 has incorrect exon/intron structure.
Comments: The annotation in the genomic sequence for nubbin is way off, as is
the predicted coding sequence and predicted protein. Using the coordinates of
the sequence scaffold that contains nubbin that is returned at NCBI if you do
a fly genomic blast with nubbin sequence, AE003636, the correct intron/exon
structure should be:
(coordinates from AE003636)
exon 1: 220761-221102 AGTTT...AATCGATgtaa.... (exon in caps, intron
lowercase)
exon 2: 224952-224956 tttcagATATCA...GGATGgtaa...
exon 3: 227432-229160 ttccagCTTCC...CAGCAGgtgt...
exon 4: 229401-230110 cttgcagGTTC...TTAATATCAA
Note: the first ATG in the nub ORF is at 220973; the start site of exon 1 was
derived from the longest embryonic cDNA clone sequence and confirmed by primer
extension and RNase protection.
Ng, M., Diaz-Benjumea, F.J., Cohen, S.M reported alternative first exon and
splicing in the wing disk, but gave no sequence data.