Gene or accession: CG9414
Gene annotation error
Gene CG9414 corresponds to 'dCAD'
Comments: The gene referred to as 'dCAD' in Yokoyama et al., 2000, J. Biol.
Chem. 275(17): 12978-12986 corresponds to CG9414 (Rep4, FBgn0028406).
In the above paper 'dCAD' is said to correspond to EST HL03573.
BLAST of the 'Drosophila genome' dataset at the NCBI with the HL03573 sequence
(AA697985) gives only 1 significant alignment,
which corresponds to part of the sequence of CG9414.
Clustal alignment of the the HL03573 sequence (AA697985) and CG9414 sequence
follows,
Subject: FB-error report: CG9414
Hi,
here is the alignment of CG9414 (Rep4, FBgn0028406) and HL03573
sequence (AA697985) as referred to in FB-error report I just sent
CLUSTAL W (1.8) Multiple Sequence Alignments
Sequence format is Pearson
Sequence 1: AA697985.HL03573 793 bp
Sequence 2: Rep4|FBgn0028406|CT26706|FBan0 1353 bp
Start of Pairwise alignments
Aligning...
Sequences ( 1:2 ) Aligned. Score: 99
Guide tree file created:
/net/nfs0/vol1/production/w3nobody/tmp/9284956681146.dnd
Start of Multiple Alignment
There are 1 groups
Aligning...
Group 1: Sequences: 2  Score:15007 
Alignment Score 5734
CLUSTAL-Alignment file created
/net/nfs0/vol1/production/w3nobody/tmp/9284956681146.aln
CLUSTAL W (1.8) multiple sequence alignment
AA697985.HL03573
\--------------------------------------------------
Rep4|FBgn0028406|CT26706|FBan0
ATGATCAGCTACATTAGAGATGCAATCATGTCACCGACAACACGCAGCAG 50
AA697985.HL03573
\--------------------------------------------------
Rep4|FBgn0028406|CT26706|FBan0
CTCGAATAACAAGAAAAGCAGCCAGCCAACAGGCGAAGTTCTCAAGGATG 100
AA697985.HL03573
\--------------------------------------------------
Rep4|FBgn0028406|CT26706|FBan0
TGAGCTCATCCGAATCCAGGGGAACAGCAGGTGCTCCAATGCGTGGCTAC 150
AA697985.HL03573
\----------ACAACGAGCGAACGAGGAAATACGGAATCGGAGCCAATTC 40
Rep4|FBgn0028406|CT26706|FBan0
AAGGTTACTGACAACGAGCGAACGAGGAAATACGGAATCGGAGCCAATTC 200
AA697985.HL03573
CCTGGAAATGCTGATAGCCAAGGCAAAAAGCAAGTTCCCGCTTCTCGAGC 90
Rep4|FBgn0028406|CT26706|FBan0
CCTGGAAATGCTGATAGCCAAGGCAAAAAGCAAGTTCCCGCTTCTCGAGC 250
AA697985.HL03573
CACATTTGTATTTGGCTTCTGATGGCTTCGAGGTGTCCGACGATGAGTAC 140
Rep4|FBgn0028406|CT26706|FBan0
CACATTTGTATTTGGCTTCTGATGGCTTCGAGGTGTCCGACGATGAGTAC 300
AA697985.HL03573
TTAAAAAGCCTACCCGCCCAGACGCTGTTCATAGTGTCTGGCCCGGATGC 190
Rep4|FBgn0028406|CT26706|FBan0
TTAAAAAGCCTACCCGCCCAGACGCTGTTCATAGTGTCTGGCCCGGATGC 350
AA697985.HL03573
AGTCATTACAACAGATGCCGATTTTGAGTTTGAGAAGATGCGACAACAAT 240
Rep4|FBgn0028406|CT26706|FBan0
AGTCATTACAACAGATGCCGATTTTGAGTTTGAGAAGATGCGACAACAAT 400
AA697985.HL03573
CGCCTTTGCTAAAGGTGGCTGACATTTTCTACGATTTTATCGAACAGCAT 290
Rep4|FBgn0028406|CT26706|FBan0
CGCCTTTGCTAAAGGTGGCTGACATTTTCTACGATTTTATCGAACAGCAT 450
AA697985.HL03573
CCGGAAAAGTTCCGCCGCATGATAACGGAGTATGAACACCAAAAGCAGCG 340
Rep4|FBgn0028406|CT26706|FBan0
CCGGAAAAGTTCCGCCGCATGATAACGGAGTATGAACACCAAAAGCAGCG 500
AA697985.HL03573
ACGCGTCCTGGATAACAGCAAGACCCACCTCAGCTTAAAGGCCGAGCACG 390
Rep4|FBgn0028406|CT26706|FBan0
ACGCGTCCTGGATAACAGCAAGACCCACCTCAGCTTAAAGGCCGAGCACG 550
AA697985.HL03573
TGGAGTGGTTCACGGGCGGCGAGGAGC-CTTCCACTCTAAGGAGGAAGCT 439
Rep4|FBgn0028406|CT26706|FBan0
TGGAGTGGTTCACGGGCGGCGAGGAGCGCTTCCACTCTAAGGAGGAAGCT 600
AA697985.HL03573
ATGGCCACACGTGCCCAGACAACGTGTGCGAGGCTACTACTATAAGGCCA 489
Rep4|FBgn0028406|CT26706|FBan0
ATGGCCACACGTGCCCAGACA-CGTGTGCGAGGCTACTACTATAAGGCCA 649
AA697985.HL03573
AGGAGGAGCTGACCCGCAATCCCTTGTACCGCCAAAATGCCAAGGCCCGA 539
Rep4|FBgn0028406|CT26706|FBan0
AGGAGGAGCTGACCCGCAATCCCTTGTACCGCCAAAATGCCAAGGCCCGA 699
AA697985.HL03573
CAAGTGATAAACTCTGTGCTGGAGAAGTTTCGATACCTGCTCATCGGTTG 589
Rep4|FBgn0028406|CT26706|FBan0
CAAGTGATAAACTCTGTGCTGGAGAAGTTTCGATACCTGCTCATCGGTTG 749
AA697985.HL03573
CGACTTTTTCTCCATGATGTTTGACCGGAATTGCAAGCAAAAGCATGAAT 639
Rep4|FBgn0028406|CT26706|FBan0
CGACTTTTTCTCCATGATGTTTGACCGGAATTGCAAGCAAAAGCATGAAT 799
AA697985.HL03573
TCCTGAAGCAGCACTTGGGCGACGAGGAAACAGACGCTGGCAGGATACCC 689
Rep4|FBgn0028406|CT26706|FBan0
TCCTGAAGCAGCACTTGGGCGACGAGGAAACAGACGCTGGCAGGATACCC 849
AA697985.HL03573
AGCAAGAGACTGAGGCAGGTGATTAGGGAGTACACCAAGGAAAACTGCAT 739
Rep4|FBgn0028406|CT26706|FBan0
AGCAAGAGACTGAGGCAGGTGATTAGGGAGTACACCAAGGAAAACTGCAT 899
AA697985.HL03573
CCTCGACGAATGGTCCACTTCCTTGTGCTCCGACTTGGGTGACTTCTATT 789
Rep4|FBgn0028406|CT26706|FBan0
CCTCGACGAATGGTCCACTTCCTTGTGCTCCGACTTGGGTGACTTCTATT 949
AA697985.HL03573
GCCA---------------------------------------------- 793
Rep4|FBgn0028406|CT26706|FBan0
GCCAGGGCTCCTATTCGGAGAATGGCAACAGCTGCTCTAAGCAGCACACT 999
AA697985.HL03573
\--------------------------------------------------
Rep4|FBgn0028406|CT26706|FBan0
ATTAATCCGTACGCTTCGCGTGAGAATCTCATTTTGTTTCAGGTCTGGAA 1049
AA697985.HL03573
\--------------------------------------------------
Rep4|FBgn0028406|CT26706|FBan0
TCTGGACCACCAAATCGAACTGTGCCGCACAATCCTTCCCGCACTTGTTG 1099
AA697985.HL03573
\--------------------------------------------------
Rep4|FBgn0028406|CT26706|FBan0
CAAATGTGGAAGAACTTGTGAGTCATCCGCAGACCAAGTGTTCAATTCAT 1149
AA697985.HL03573
\--------------------------------------------------
Rep4|FBgn0028406|CT26706|FBan0
AAGAAGCAAGTGGTCGATATCTCAGTACTGGAGTACTTTCTAGAAATATT 1199
AA697985.HL03573
\--------------------------------------------------
Rep4|FBgn0028406|CT26706|FBan0
CTCCCTAAAGAATCTCAAACTAGTGCACATTGTCTGTCATGAGAAGGCGC 1249
AA697985.HL03573
\--------------------------------------------------
Rep4|FBgn0028406|CT26706|FBan0
AGCGCTCAAACCGTTCGAATGGTCGCCTACTGTGCTCCGACTGTCATGAG 1299
AA697985.HL03573
\--------------------------------------------------
Rep4|FBgn0028406|CT26706|FBan0
TATCGCATTGTGCAAGAGCTGATGGAAGTCCAGGTGGACAGTCTAGCCAC 1349
AA697985.HL03573 \----
Rep4|FBgn0028406|CT26706|FBan0 TTAA 1353