Gene or accession: CG12276
Gene annotation error
Gene CG12276 corresponds to AF193554 (FBgn0029512)
Comments: CG12276|CT17770| corresponds to Aos1 (FBgn0029512). ClustalW
alignments of
the Aos1 and CG12276|CT17770| CDSs are being sent to flybase-updates in a
separate message.
Subject: Aos1-CG12276|CT17770 ClustalW alignment
Aos1 corresponds to CG12276.
Aos1 and CG12276|CT17770 ClustalW alignment.
Pairwise Scores:
CLUSTAL W (1.8) Multiple Sequence Alignments
Sequence format is Pearson
Sequence 1: gi|6694276|gb|AAF25198.1|AF193 337 aa
Sequence 2: gi|7299631|gb|AAF54815.1| 337 aa
Start of Pairwise alignments
Aligning...
Sequences ( 1:2 ) Aligned. Score: 99
Guide tree file created:
/net/nfs0/vol1/production/w3nobody/tmp/1047959028373.dnd
Start of Multiple Alignment
There are 1 groups
Aligning...
Group 1: Sequences: 2  Score:5488 
Alignment Score 2029
CLUSTAL-Alignment file created
/net/nfs0/vol1/production/w3nobody/tmp/1047959028373.aln
CLUSTAL W (1.8) multiple sequence alignment
gi|6694276|gb|AAF25198.1|AF193
MVVDMDTSETAVELTEAENELYDRQIRLWGLESQKRLRTAKILIAGLCGL 50
gi|7299631|gb|AAF54815.1|
MVVDMDTSETAVELTEAENELYDRQIRLWGLESQKRLRTAKILIAGLCGL 50
gi|6694276|gb|AAF25198.1|AF193
GAEITKNIILSGVNSVKLLDDKDVTEEDFCSQFLVPRESLNTNRAEASLT 100
gi|7299631|gb|AAF54815.1|
GAEITKNIILSGVNSVKLLDDKDVTEEDFCSQFLVPRESLNTNRAEASLT 100
gi|6694276|gb|AAF25198.1|AF193
RARALNPMVDISADREPLKEKTSEFFGQFDVVVVNGATNEELLRIDTICR 150
gi|7299631|gb|AAF54815.1|
RARALNPMVDISADREPLKEKTSEFFGQFDVVVVNGATNEELLRIDTICR 150
gi|6694276|gb|AAF25198.1|AF193
DLGVKFIATDVWGTFGFYFASLQKHSYVEDVIKHKVVANSEKKKKYETVS 200
gi|7299631|gb|AAF54815.1|
DLGVKFIATDVWGTFGFYFASLQKHSYVEDVIKHKVVANSEKKKKYETVS 200
gi|6694276|gb|AAF25198.1|AF193
IPTQRDVDYPGYSAWLDFDVTEPSYLRKLKRNGPGVLLLSVLQKFRTTHK 250
gi|7299631|gb|AAF54815.1|
IPTQRDVDYPGYSAWLDFDVTEPSYLRKLKRNGPGVLLLSVLQKFRTTHK 250
gi|6694276|gb|AAF25198.1|AF193
RDPSYKTREADLELLRGIRDELLPNSILGNEALGLIFAQISPAVAVVGGV 300
gi|7299631|gb|AAF54815.1|
RDPSYKTREADLELLRGIRDELLPNSILGDEALGLIFAQISPAVAVVGGV 300
gi|6694276|gb|AAF25198.1|AF193 VAQEVIKVVTKLEAPHRNLFVFDPETCAGYVEAIGAK 337
gi|7299631|gb|AAF54815.1| VAQEVIKVVTKLEAPHRNLFVFDPETCAGYVEAIGAK 337
Subject: Aos1 and SAE1 (plus CG12276)
Michael and the Cambridge curators,
By alignment, Aos1 and SAE1 seem to be the same gene.
The attached file contains the BLASTN and ClustalW alignments
for the nucleotide and protein sequences for Aos1 and SAE1.
I have just sent in a FB-updates/error report indicating
that Aos1 corresponds to CG12276|CT17770.
Leyla
================================================================================
SAE1 AF218863 AAF31703.1
Aos1 AF193554 AAF25198.1
SAE1 and Aos1 BLASTN alignment
Sequence 1 gi 6934293 Length 1169 (1 .. 1169) (SAE1)
Sequence 2 gi 6694275Length 1169 (1 .. 1169) (Aos1)
Score = 2173 bits (1130), Expect = 0.0
Identities = 1134/1136 (99%), Positives = 1134/1136 (99%)
Query: 4
ctgaaagttgttgcaagaaatttgtattaagttgtgaaaaatacattttaaacttgataa 63
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 9
ctgaaagttgttgcaagaaatttgtattaagttgtgaaaaatacattttaaacttgataa 68
Query: 64
ataaataatggtcgtggatatggacaccagcgagacagccgtggagctcactgaggcgga 123
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 69
ataaataatggtcgtggatatggacaccagcgagacagccgtggagctcactgaggcgga 128
ubiquitin-like protein activati> 1 M V V D M D T S E T A
V E L T E A E
Query: 124
gaacgagctgtacgacagacaaatccgactctggggtctggaatctcagaaacgccttcg 183
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 129
gaacgagctgtacgacagacaaatccgactctggggtctggaatctcagaaacgccttcg 188
ubiquitin-like protein activati> 19 N E L Y D R Q I R L W G L
E S Q K R L R
Query: 184
cacggccaagattctcatcgctggactgtgcggcctgggcgccgagataaccaagaacat 243
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 189
cacggccaagattctcatcgctggactgtgcggcctgggcgccgagataaccaagaacat 248
ubiquitin-like protein activati> 39 T A K I L I A G L C G L G
A E I T K N I
Query: 244
catcctgtccggagtgaactccgtgaagctgctggatgacaaggacgtaaccgaggagga 303
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 249
catcctgtccggagtgaactccgtgaagctgctggatgacaaggacgtaaccgaggagga 308
ubiquitin-like protein activati> 59 I L S G V N S V K L L D D
K D V T E E D
Query: 304
cttttgttcacaattccttgtcccccgtgaatcgctgaacaccaaccgggccgaagcatc 363
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309
cttttgttcacaattccttgtcccccgtgaatcgctgaacaccaaccgggccgaagcatc 368
ubiquitin-like protein activati> 79 F C S Q F L V P R E S L N
T N R A E A S
Query: 364
attgacacgggcacgtgctctcaatcccatggtggacatctccgccgaccgcgagccctt 423
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 369
attgacacgggcacgtgctctcaatcccatggtggacatctccgccgaccgcgagccctt 428
ubiquitin-like protein activati> 99 L T R A R A L N P M V D I
S A D R E P L
Query: 424
gaaggagaagacctctgagttcttcggtcagttcgacgttgtggtggtcaatggcgcgac 483
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 429
gaaggagaagacctctgagttcttcggtcagttcgacgttgtggtggtcaatggcgcgac 488
ubiquitin-like protein activati> 119 K E K T S E F F G Q F D V
V V V N G A T
Query: 484
caacgaggaactgttgcgcattgacaccatttgccgggacctgggcgtcaagttcatagc 543
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 489
caacgaggaactgttgcgcattgacaccatttgccgggacctgggcgtcaagttcatagc 548
ubiquitin-like protein activati> 139 N E E L L R I D T I C R D
L G V K F I A
Query: 544
caccgatgtgtggggcaccttcgggttctactttgccagtctgcagaagcacagctacgt 603
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 549
caccgatgtgtggggcaccttcgggttctactttgccagtctgcagaagcacagctacgt 608
ubiquitin-like protein activati> 159 T D V W G T F G F Y F A S
L Q K H S Y V
Query: 604
cgaggatgttattaatcacaaggtcgtagccaattcggagaagaaaaagaagtatgaaac 663
|||||||||||||||
||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 609
cgaggatgttattaagcacaaggtcgtagccaattcggagaagaaaaagaagtatgaaac 668
ubiquitin-like protein activati> 179 E D V I K H K V V A N S E
K K K K Y E T
Query: 664
cgtgtccattccaacgcagcgtgatgtggattatcccggctactctgcctggcttgattt 723
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 669
cgtgtccattccaacgcagcgtgatgtggattatcccggctactctgcctggcttgattt 728
ubiquitin-like protein activati> 199 V S I P T Q R D V D Y P G
Y S A W L D F
Query: 724
cgatgttaccgagcctagttatttgcgaaaattgaagcgcaatggcccgggtgttttgct 783
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 729
cgatgttaccgagcctagttatttgcgaaaattgaagcgcaatggcccgggtgttttgct 788
ubiquitin-like protein activati> 219 D V T E P S Y L R K L K R
N G P G V L L
Query: 784
attaagtgtcctgcaaaagttccgcacaacccacaagcgggatcccagctacaagacccg 843
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 789
attaagtgtcctgcaaaagttccgcacaacccacaagcgggatcccagctacaagacccg 848
ubiquitin-like protein activati> 239 L S V L Q K F R T T H K R
D P S Y K T R
Query: 844
agaagctgacctggaattgcttcgcggaattcgcgacgaactgctgcccaactctatact 903
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 849
agaagctgacctggaattgcttcgcggaattcgcgacgaactgctgcccaactctatact 908
ubiquitin-like protein activati> 259 E A D L E L L R G I R D E
L L P N S I L
Query: 904
gggcgacgaagctctaggtctgatttttgcacagatctcgccagcggtcgctgttgtcgg 963
||||
|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 909
gggcaacgaagctctaggtctgatttttgcacagatctcgccagcggtcgctgttgtcgg 968
ubiquitin-like protein activati> 279 G N E A L G L I F A Q I S
P A V A V V G
Query: 964
cggcgttgtggcacaagaggtcatcaaggtggttactaaattggaggcgccacaccgtaa 1023
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 969
cggcgttgtggcacaagaggtcatcaaggtggttactaaattggaggcgccacaccgtaa 1028
ubiquitin-like protein activati> 299 G V V A Q E V I K V V T K
L E A P H R N
Query: 1024
tctgttcgtttttgacccagagacttgtgccggatacgttgaggccatcggagcgaagtg 1083
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1029
tctgttcgtttttgacccagagacttgtgccggatacgttgaggccatcggagcgaagtg 1088
ubiquitin-like protein activati> 319 L F V F D P E T C A G Y V
E A I G A K ^^
Query: 1084
aatttaccacaaagggcttatttataaaaatctgaatatcaaataacaccagttgt 1139
||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1089
aatttaccacaaagggcttatttataaaaatctgaatatcaaataacaccagttgt 1144
ubiquitin-like protein activati> 338 ^
CPU time: 0.11 user secs. 0.02 sys. secs 0.13 total secs. Gapped Lambda K H
1.33 0.621 1.12 Matrix: blastn  matrix:1  \-2 Gap Penalties: Existence: 5,
Extension: 2 Number of Hits to DB: 1
Number of Sequences: 0 Number of extensions: 1 Number of successful
extensions: 1 Number of sequences better than 10.0: 1 length of query: 1169
length of database: 706,884,452
effective HSP length: 23 effective length of query: 1146 effective length of
database: 692,976,559 effective search space: 794151136614 effective search
space used: 794151136614 T: 0 A: 0
X1: 6 (11.5 bits) X2: 26 (50.0 bits) S1: 12 (23.8 bits) S2: 19 (37.2 bits)
_______________________________________________________________________
CLUSTAL W (1.8) Multiple Sequence Alignments
Sequence format is Pearson
Sequence 1: gi|6694276|gb|AAF25198.1|AF193 337 aa
Sequence 2: gi|6934294|gb|AAF31703.1|AF218 337 aa
Start of Pairwise alignments
Aligning...
Sequences ( 1:2 ) Aligned. Score: 99
Guide tree file created:
/net/nfs0/vol1/production/w3nobody/tmp/23494959027282.dnd
Start of Multiple Alignment
There are 1 groups
Aligning...
Group 1: Sequences: 2  Score:5480 
Alignment Score 2024
CLUSTAL-Alignment file created
/net/nfs0/vol1/production/w3nobody/tmp/23494959027282.aln
Your guide tree:
23494959027282.dnd
(gi|6694276|gb|AAF25198.1| AF193:0.00297 ,gi|6934294|gb|AAF31703.1| AF218:0.00297 );
Your Multiple Sequence Alignment:
23494959027282.aln
CLUSTAL W (1.8) multiple sequence alignment
gi|6694276|gb|AAF25198.1|AF193
MVVDMDTSETAVELTEAENELYDRQIRLWGLESQKRLRTAKILIAGLCGL 50
gi|6934294|gb|AAF31703.1|AF218
MVVDMDTSETAVELTEAENELYDRQIRLWGLESQKRLRTAKILIAGLCGL 50
gi|6694276|gb|AAF25198.1|AF193
GAEITKNIILSGVNSVKLLDDKDVTEEDFCSQFLVPRESLNTNRAEASLT 100
gi|6934294|gb|AAF31703.1|AF218
GAEITKNIILSGVNSVKLLDDKDVTEEDFCSQFLVPRESLNTNRAEASLT 100
gi|6694276|gb|AAF25198.1|AF193
RARALNPMVDISADREPLKEKTSEFFGQFDVVVVNGATNEELLRIDTICR 150
gi|6934294|gb|AAF31703.1|AF218
RARALNPMVDISADREPLKEKTSEFFGQFDVVVVNGATNEELLRIDTICR 150
gi|6694276|gb|AAF25198.1|AF193
DLGVKFIATDVWGTFGFYFASLQKHSYVEDVIKHKVVANSEKKKKYETVS 200
gi|6934294|gb|AAF31703.1|AF218
DLGVKFIATDVWGTFGFYFASLQKHSYVEDVINHKVVANSEKKKKYETVS 200
gi|6694276|gb|AAF25198.1|AF193
IPTQRDVDYPGYSAWLDFDVTEPSYLRKLKRNGPGVLLLSVLQKFRTTHK 250
gi|6934294|gb|AAF31703.1|AF218
IPTQRDVDYPGYSAWLDFDVTEPSYLRKLKRNGPGVLLLSVLQKFRTTHK 250
gi|6694276|gb|AAF25198.1|AF193
RDPSYKTREADLELLRGIRDELLPNSILGNEALGLIFAQISPAVAVVGGV 300
gi|6934294|gb|AAF31703.1|AF218
RDPSYKTREADLELLRGIRDELLPNSILGDEALGLIFAQISPAVAVVGGV 300
gi|6694276|gb|AAF25198.1|AF193 VAQEVIKVVTKLEAPHRNLFVFDPETCAGYVEAIGAK 337
gi|6934294|gb|AAF31703.1|AF218 VAQEVIKVVTKLEAPHRNLFVFDPETCAGYVEAIGAK 337