Gloor, G.B., Moretti, J., Mouyal, J., Keeler, K.J. (2000). Distinct P-element excision products in somatic and germline cells of Drosophila melanogaster. Genetics 155(4): 1821--1830.
FlyBase ID
FBrf0129827
Publication Type
Research paper
Abstract
The footprints remaining following somatic P-element excision from the Drosophila white locus were recovered and characterized. Two different types of footprints were observed. Over 75% of the footprints were short, composed of 4 or 7 nucleotides of the P-element inverted terminal repeat, and were similar to those found in a previously described plasmid excision assay. The remaining footprints were composed of 14-18 nucleotides of both inverted terminal repeats. These large footprints were indistinguishable from those recovered following germline P-element excision. Enhanced expression of the Drosophila homologue of the Ku70 protein did not affect the structure of the somatic footprints. Therefore, this protein is not a limiting factor for double-strand break repair by nonhomologous end-joining in Drosophila somatic cells.
