Subject: Re: FlyBase Query (cy729)
>Dear Dr Klaembt,
>
>I sent you a Query a few weeks ago. I was wondering if you have had a
>chance to look at it yet? I enclose the email for your convenience.
>Sorry I had completely forgotten to answer your email.
>Hummel et al, 2000, Neuron 26(2): 357-370.
>
>I have a few questions I was hoping you could answer for me.
>
..
>2. ftz-GAL4 and 44-GAL4
>=======================
>You mention 2 GAL4 lines in your paper, ftz-GAL4 and 44-GAL4, that you
>state as being unpublished. Could you provide me with a few details
>about these lines? Are they enhancer trap or promoter fusion lines?
>3. P0163
>========
>You mention a GAL4 line P0163. Can you tell me what transposon was
>used in your screen to generate this line?
all lines are enhancer trap lines generated with the P<up>GAL4, white</up>
transposon (X-chr.) from Andrea Brand.
We have checked by in situ hybridization to polytene chr. that ftz-GAL
carries an insertion in the ftz region.
i hope this answers your questions,
best wishes christian>
>
>Any new information that you give to me will be curated as a personal
>communication from you to FlyBase, if thats O.K. with you.
>
>Best wishes,
>
>Chihiro
>
>----------------------------------------------------------------------
>Chihiro Yamada.
>
>FlyBase (Cambridge),
>----------------------------------------------------------------------
>
>----- End Included Message \-----
Dr. Christian Klämbt
Institut fuer Neurobiologie
Universität Münster
Badestrasse 9
D-48149 Münster Germany
====
Subject: FlyBase Query (cy729)
Dear Dr Klaembt,
I am currently curating your paper for FlyBase:
Hummel et al, 2000, Neuron 26(2): 357-370.
I have a few questions I was hoping you could answer for me.
1. UAS-MAP2c
============
In fig.6 you mention a UAS-MAP2c line. there is only one such
construct in our records, could you confirm that this the same
construct as the one I describe below? If it is not, could you give me
some information about this line please? Where did you get this line
from? Do you have any of the molecular details of this construct?
Rnor\MAP2Scer\UAS.cAa
Adam, 1996.1.17, personal communication to FlyBase
Warrick et al., 1999, Nature Genetics 23(4): 425--428
P{UAS-MAP2.A}
UAS sequences direct the expression of Rnor\MAP2 c-transcript
coding region.
2. ftz-GAL4 and 44-GAL4
=======================
You mention 2 GAL4 lines in your paper, ftz-GAL4 and 44-GAL4, that you
state as being unpublished. Could you provide me with a few details
about these lines? Are they enhancer trap or promoter fusion lines?
If they are the former, could you tell me what transposon was used to
generate the lines? If they are the latter could you give me some
molecular details for these constructs?
3. P0163
========
You mention a GAL4 line P0163. Can you tell me what transposon was
used in your screen to generate this line?
Any new information that you give to me will be curated as a personal
communication from you to FlyBase, if thats O.K. with you.
Best wishes,
Chihiro
\----------------------------------------------------------------------
Chihiro Yamada.
FlyBase (Cambridge),
\----------------------------------------------------------------------