Dear Flybase,
We have identified two new non-LTR retrotransposon in the genome of
Drosophila melanogaster, Waldo-A and Waldo-B. They are described in an article
which is in press in Mol. Biol. Evol.:
Busseau I, Berezikov E and Bucheton A (2001) Identification of Waldo-A and
Waldo-B, two closely related non-LTR retrotransposons in Drosophila.
Mol. Biol. Evol., in press.
We think that it would be useful to the Drosophila community that these
elements appear in the databases. We are not sure about the way to do this.
You will find attached two rtf files indicating the coordinates of the Waldo-A
and Waldo-B elements in GenBank sequences accession numbers AC005734 and
AC005847, that were produced by the BDGP. Please let me know if these files
are useful to you, if we should provide additional information, if we should
contact directly GenBank and/or BDGP.
Sincerely yours, I Busseau.
\------------------------------------------------------------------
Isabelle Busseau
IGH CNRS
141 rue de la Cardonille
34396 Montpellier cedex 5
\------------------------------------------------------------------
We have identified a new non-LTR retrotransposon in the genome of Drosophila
melanogaster. We have named it Waldo-A.
Reference:
Busseau I, Berezikov E and Bucheton A (2001) Identification of Waldo-A and
Waldo-B, two closely related non-LTR retrotransposons in Drosophila.
Mol. Biol. Evol., in press.
The Waldo-A element was identified by analyzing the sequence in GenBank
accession number AC005734. Coordinates: 107957-102787.
107963-107958: putative Target Site Duplication.
107575-106079: ORF1. Similar to retrovirus gag proteins with cysteine-rich
motifs; possible nucleic acid binding protein. Translation:
MDSAVGGSPPDRNDPFRRSPRLSRSPTRGVGNPTHGDGISVPSVARGEHA
GPQPPLTSDCSDAVEVENLAPTASTTATSQKTMDHIAVPSQKTGGKSPAG
SPHRSSDLTAALQNEDLRGILTRMNGKISTLLSAFETQRHVTRETKGIAS
ELSALNNRALELYKGTTSEHRLKSSATQTEVQKPTKKAPQNAQVPKVQVP
KIQAPKPSNPTLGGNGEEKATSRPNESKPGSYASVAKNASTGVEWTKVKP
TRLRKKPEALIVKKTGEASYAEMLRKLRSDPSLSELGSHVRKIRRTQKGE
LLLEVEGKASESVPKFKSDLEAALNDLASVRTGAQRIALSCSGLDEATTA
EELHSCLVAQFQGLQINPEDIRGLRRMRDGTQIASVLLNANVAIPVLKQG
TITVGWSRCRITQDVRPTRCYRCLGYGHRSATCKNTDRADCCLRCGERGH
KAKGCVAAPKCLICSSEVDRNHSTGSFACPTYRATLKEAKSHLNAHSY
106095-103132: ORF2. Probable reverse transcriptase. Translation:
MHTHISVVQLNVNHCAAAQSLLAQTAAERNVDIMLLSEPYVSGSGQSSMI
LDETGKAAIKCCSSLHVEELAALPMRGIAYAKLKHVHLYSCYAPPSDTPD
QFEEFLEALVDHARGRSPKVIAGDFNAWAVEWGSRTSNTRGRAVIDAMGM
LDLILLNDGRKPTFNNDRGTSFIDVTFVSRGLVDNNNWMVHDVMTLSDHA
LISFSLSPEDMPRRRQSRAVGKAWDTRKIDEAMLAYQINSLEIPSGDAES
MAAGLMNMLGRICDAIMPRKNKAQRKPPVYWWSASLSQLRSDCLRARRMA
QRARGSTHHAELLEAFRRKRLEFKHGIAAAKARSFKELQDGVDSDTWGLA
YKLVTKKLRRRAATPSDPGVLANIVGELFPKQTTLWRPTEAAPAPDFPCV
TELEVAEAAKRIKPNKAPGLDGIPGAVIKAVALGRPEIFRATFQQCLLDG
IFPTRWKSQKLVLLPKGKGPAHAANSYRPLCLLDIVGKLFERILYTRIEA
ITESINGLGSHQYGFRKGKSTLDALSAVCNIAKTAISGDRWLGGRKEYCA
IVTLDVRNAFNTARWPVILAAMYRMGIPEYLRIVVGSYFRDRVLWYDTED
GPKRYRVSAGVPQGSVLGPILWNIMYDGILGINRPVGVELHCFADDVAIT
AVSKTIAGLEDKCNSTIGAAIRWLEKAGLAIAAHKTEAVLLSSRKKVENM
LVSVKGTQVTSQESLKYLGVMIDRRLSFKDHASHASKKAAITASSLARLM
PNVGGPRHPARKLLVSVAKASLLYAAPVWSNATGRVSYLKGARSVLRSMS
LRLIRGFRTISEDAALALAGLPPIDLEIKALSLMRSGASRQEAHEWLLGE
WQSRWQTSRRGRWTYQLIPEMTVWAECQHKCLDYHLTQFLTDHGCFRAYL
LRFRHVESAQCLFCVDGEETAEHVLMHCSRFTAEREQLKTLSGSPFSPSG
LFAAMMANRGAWERGHSIIINMMKRVRSDEMANRVDV
102786-102781: putative Target Site Duplication.
Full sequence of the Waldo-A element (5171 bp):
GCTGCCAACTTGGCGCAGCGGGCGATCTGGAGAGGGAGGCGTTGCCAGAT
CAACCGTGTGATCAGTGTTGTGTTGCGACAGTAGAGCGCGAAATTCAAAA
TTAAACTTAAAATTAAACCGTTGAATTTTAATTTAATTTTAATTTTGATT
CTGGTTACTGGGAGCGCACAAGTCTTTAAGAAAGGGGAAAAGGCACAAAG
GTCGAGGACAGACCAAATATCATCCTCTCACCGTGCTGGTGCCTAAGCAG
AGGCTCTGGTGTCCGAGTCGAGGCGGTACAAACCTCCATCCGTAAAAGCC
AGGGGGAAATCCGTGGCCTACGTGGGAACCCCTTTCTAAGGCTGGTGCTT
CGCCTAGGTAACCTTAGGCCATCTTTCAGTTGATGGACAGTGCAGTGGGG
GGCAGTCCCCCTGATCGGAACGACCCGTTCAGGAGGAGCCCCCGACTTTC
GAGATCCCCCACCAGAGGAGTCGGTAACCCGACCCATGGTGACGGAATCT
CGGTCCCGTCGGTGGCAAGGGGTGAGCACGCAGGCCCCCAGCCGCCGTTG
ACTAGCGACTGCAGCGATGCTGTAGAGGTGGAGAACTTGGCTCCTACAGC
ATCTACGACCGCGACAAGTCAGAAGACGATGGACCACATCGCCGTGCCAT
CGCAAAAGACGGGAGGGAAGTCCCCAGCTGGTTCCCCTCATCGGTCTTCG
GACCTGACCGCGGCTCTCCAGAATGAGGACCTGAGGGGCATACTTACCAG
GATGAATGGCAAGATCAGCACCTTGCTATCCGCATTCGAGACCCAACGGC
ACGTGACACGAGAGACAAAGGGCATAGCTTCCGAACTCTCAGCCCTTAAC
AACAGGGCGTTAGAGCTGTACAAAGGTACTACTAGCGAGCACCGCTTGAA
GAGCAGTGCTACCCAGACGGAGGTACAAAAGCCTACCAAAAAGGCTCCTC
AAAATGCACAGGTACCCAAGGTGCAAGTGCCAAAAATCCAGGCGCCCAAG
CCGAGCAACCCTACGTTGGGTGGCAACGGTGAAGAAAAGGCAACATCCAG
ACCAAACGAGTCCAAACCAGGCAGCTACGCATCTGTCGCCAAGAATGCTA
GCACGGGTGTAGAGTGGACCAAGGTGAAGCCTACGCGCCTGCGTAAAAAA
CCGGAGGCACTCATCGTAAAAAAGACAGGAGAGGCTTCGTACGCAGAGAT
GCTTCGGAAGCTAAGATCGGACCCGAGCCTTAGCGAACTGGGCAGCCACG
TGCGAAAAATCCGGAGAACGCAGAAAGGTGAGCTGTTGCTCGAGGTAGAG
GGGAAAGCTTCGGAAAGCGTCCCCAAGTTTAAGAGCGACCTGGAAGCGGC
GCTCAATGACTTGGCCTCTGTGCGCACAGGAGCGCAAAGAATAGCTCTAT
CTTGCAGCGGATTGGACGAGGCTACGACAGCAGAGGAGCTCCACAGCTGC
TTGGTCGCCCAATTCCAGGGCCTGCAGATAAATCCTGAAGATATCAGGGG
CCTTCGCAGAATGCGGGATGGCACGCAAATAGCCTCAGTGCTGCTGAACG
CGAACGTTGCGATACCAGTCCTTAAACAGGGCACCATAACCGTTGGATGG
TCAAGATGTCGTATCACCCAGGACGTTCGACCCACGAGATGCTACAGGTG
TCTCGGCTATGGGCATCGATCAGCAACCTGCAAGAACACTGACAGGGCAG
ACTGCTGTCTTAGATGCGGTGAGCGTGGGCACAAGGCAAAGGGGTGCGTT
GCAGCACCAAAATGCCTGATCTGCAGCAGCGAGGTGGACAGAAACCACTC
GACGGGTAGCTTTGCGTGCCCGACCTACAGAGCGACCCTAAAAGAAGCCA
AGAGCCACCTTAATGCACACTCATATTAGCGTAGTACAGCTCAATGTCAA
TCATTGCGCAGCAGCTCAGAGCCTCCTGGCCCAGACTGCGGCTGAGCGCA
ATGTAGACATCATGCTCCTAAGCGAACCCTACGTCTCTGGTAGCGGACAA
TCGTCCATGATCCTTGACGAGACAGGTAAAGCAGCTATCAAATGCTGCAG
CTCTCTCCACGTCGAGGAACTGGCTGCTTTACCTATGCGGGGTATCGCTT
ATGCGAAGTTAAAACACGTGCACTTGTACAGCTGCTACGCTCCGCCGAGC
GACACCCCCGATCAGTTCGAGGAGTTTCTGGAGGCGCTCGTGGACCATGC
GAGAGGGCGAAGCCCGAAGGTCATTGCCGGCGACTTTAATGCCTGGGCAG
TGGAATGGGGCAGCAGGACATCCAACACCAGAGGCCGAGCTGTGATTGAC
GCCATGGGAATGCTGGACCTTATACTGCTGAACGACGGACGGAAGCCGAC
GTTTAACAACGATAGGGGTACGTCCTTTATTGACGTTACCTTTGTCAGCA
GAGGGCTAGTAGACAACAATAACTGGATGGTCCATGACGTCATGACGCTG
AGCGACCACGCCCTGATCTCCTTCAGTCTCTCCCCGGAGGACATGCCCAG
GAGACGGCAGAGTAGAGCAGTCGGGAAAGCATGGGACACCAGGAAGATCG
ATGAGGCCATGCTGGCCTATCAGATCAATTCCCTGGAAATCCCAAGTGGG
GACGCAGAGAGTATGGCGGCAGGCCTCATGAATATGCTGGGAAGAATCTG
CGACGCAATCATGCCAAGGAAAAATAAGGCACAGCGCAAACCACCCGTTT
ACTGGTGGAGCGCCTCCCTAAGCCAACTACGGTCTGATTGCCTCAGGGCT
AGGAGAATGGCGCAACGAGCCAGAGGCAGTACCCACCACGCGGAACTCTT
GGAGGCTTTCAGAAGGAAACGTCTAGAGTTCAAGCACGGCATCGCGGCTG
CCAAAGCGCGGTCGTTTAAGGAGCTGCAGGATGGCGTAGACAGCGATACC
TGGGGCCTCGCCTACAAGCTTGTTACCAAAAAGCTAAGGAGGAGAGCGGC
AACCCCATCCGACCCGGGGGTCCTGGCTAACATAGTAGGGGAGCTATTCC
CAAAGCAGACCACACTATGGAGGCCAACAGAGGCAGCCCCTGCCCCAGAT
TTTCCGTGCGTCACAGAACTTGAAGTCGCCGAGGCAGCCAAGCGCATCAA
ACCCAACAAAGCCCCTGGACTAGATGGTATTCCTGGAGCTGTTATAAAAG
CAGTGGCGCTGGGTAGACCTGAAATCTTCAGGGCCACCTTCCAGCAATGC
CTTCTGGACGGAATCTTCCCAACAAGGTGGAAAAGCCAGAAGCTAGTCCT
GTTGCCGAAAGGCAAGGGACCAGCACATGCTGCAAACAGCTACCGCCCTC
TATGCCTACTGGATATAGTAGGAAAACTGTTCGAACGTATCCTGTATACC
AGAATAGAGGCAATCACCGAGAGCATCAACGGCCTGGGAAGTCATCAATA
TGGCTTCCGGAAAGGTAAGAGCACTCTGGACGCTCTTTCGGCCGTTTGTA
ACATCGCCAAGACCGCTATTTCTGGTGATAGATGGTTAGGGGGCAGGAAG
GAATACTGCGCAATTGTGACTCTGGACGTAAGGAACGCTTTCAACACCGC
CAGATGGCCCGTAATCCTCGCGGCCATGTACCGTATGGGGATCCCGGAGT
ACCTAAGGATAGTCGTTGGCAGCTACTTTAGGGACCGGGTCCTATGGTAC
GATACGGAAGATGGCCCAAAAAGATACCGAGTTTCGGCAGGTGTTCCCCA
AGGATCGGTACTTGGACCAATCCTATGGAACATTATGTACGATGGGATCT
TGGGCATCAACAGGCCCGTAGGAGTAGAGCTGCATTGTTTTGCTGACGAT
GTGGCAATCACAGCTGTCTCGAAAACAATCGCAGGGTTGGAAGACAAATG
CAACTCTACGATCGGTGCTGCCATCCGCTGGCTCGAGAAAGCCGGGCTAG
CAATAGCGGCTCACAAGACCGAAGCAGTCCTACTAAGCAGCAGGAAAAAG
GTGGAGAACATGCTGGTCTCCGTCAAGGGTACACAGGTGACCTCTCAAGA
GTCCCTAAAGTACCTGGGGGTAATGATAGATCGCAGACTATCGTTCAAGG
ACCACGCGAGCCACGCCAGCAAGAAGGCAGCAATCACAGCCTCTTCGTTG
GCGAGGCTTATGCCCAACGTCGGAGGCCCAAGACACCCGGCCAGGAAACT
GCTGGTGTCAGTAGCAAAGGCTTCGCTACTATACGCTGCACCAGTCTGGA
GCAATGCCACTGGCAGGGTCTCATACCTGAAAGGAGCTCGTTCGGTGCTA
CGGTCAATGTCTCTGAGGCTCATTAGAGGTTTCAGGACCATATCCGAAGA
CGCGGCGCTAGCGCTGGCAGGCCTGCCGCCGATTGATCTGGAGATCAAGG
CTCTCAGCCTAATGCGGAGTGGCGCTTCCAGGCAAGAGGCACACGAGTGG
CTATTAGGTGAATGGCAGAGTAGATGGCAAACGTCGCGACGGGGGAGGTG
GACTTATCAGCTCATCCCAGAGATGACGGTTTGGGCAGAGTGCCAACACA
AATGCTTGGACTACCACCTAACCCAGTTCCTCACGGACCATGGCTGCTTC
CGGGCCTATCTACTCCGGTTCCGTCACGTAGAGTCAGCCCAATGCTTGTT
CTGCGTCGACGGTGAAGAAACAGCAGAACATGTGCTAATGCACTGCTCCA
GGTTCACGGCGGAGAGAGAGCAGCTAAAGACGCTGTCAGGTTCCCCGTTC
AGCCCTAGTGGCTTGTTCGCGGCTATGATGGCGAACAGGGGGGCTTGGGA
GCGGGGACACAGCATTATCATTAATATGATGAAGCGTGTCCGATCAGACG
AGATGGCCAACAGAGTGGATGTCTAAGCCCAAACTGGTGTCCTGGGTGAC
GGCGGGCGAAGAATTCATCCTCAGCGTCCCCGGCTCGTCGTAAAAGGCGA
CTAAAGGGTGGAAGGAGGAGCCCCCATGGACTACACTGAAGGAAGGGAGT
GCGACCTGGCCTCACATCCTGCTCACCGAAGTCATACCTTGACTGGCAGT
CCCGGTGAGCGAGCAAGGACTGTAGAGCACGCGGAGGTTTTTGTTTTAGT
ACGTAGGCATAATTCCAATAGGGCTTATGAATCGTGCATGCCACCTACGG
ACGGTAGGTGGTATCTTTAGAAGATTTTAATTTTCCTACCGTAAGTCAAA
TAATAAAAAAAAAAAAAAAAA
We have identified a new non-LTR retrotransposon in the genome of Drosophila
melanogaster. We have named it Waldo-B.
Reference:
Busseau I, Berezikov E and Bucheton A (2001) Identification of Waldo-A and
Waldo-B, two closely related non-LTR retrotransposons in Drosophila.
Mol. Biol. Evol., in press.
The Waldo-B element was identified by analyzing the sequence in GenBank
accession number AC005847. Coordinates: 20184-15005.
20195-20185: putative Target Site Duplication.
19750-18284: ORF1. Similar to retrovirus gag proteins with cysteine-rich
motifs; possible nucleic acid binding protein. Translation:
MDRTGGGSAPDPNDPFRRSGRLSRSPIRGVGTQIQGGGGEQAGPPPPKAS
DCSAAVEVETLATTVSTTATSLKTMDFISVPAQRAGAKSPSGSPHRSPEL
TTTLQNEDLQGILDMMKAKITAILSSFETRRHVTSEDRGVLVDLSALNKR
AIELQEGINKKPPPRSTATQTEAEKTKRSQVAPPRQALPNVQVRKTDNHR
SAAKSTGKALPTTADSKPESYASVAKDANKDEEWAKVKPKRLRKKPEALI
LKKTGEVTYSDMLRKMKAEPSLTEFGKHVRKIRRTQQGELLLELEGKASE
VIPSFKNELEATLKEIASVRTGAHRTALICSGLDETTTAQDLHNSLVSQF
QGIRLEPEDVRGLRRRRDGTQIASVLMCANDAIAVINRGVVTVGWSRCRI
AQDVRPIRCFRCLEFGHRAPYCKSVDRSDCCLRCGEHGHKAKGCVAPPRC
LICSSDVDKNHATGGFACPTYKANTKGANSRQNDARRN
18303-15334: ORF2. Probable reverse transcriptase. Translation:
MMPEEINIIQLNVNHCAAAQNLLTQTAKERHADVVLLSEPYLPGVGNSGV
LLDETGKAAIKCTSRLLVEEWDTVPMRGIAYAKIRGIHFYSCYAPPSDSP
EQFEDMLEKLVNHASGRRPTVIGGDLNAWATEWGSRISNTRGRAVIDAMN
LLDLVLLNDGFKPTFNNDRGTSFIDVTFVSRVLVAGSNWMVHEDITLSDH
NLITFGARTMRPSPKQRRCALGPVWDIRKLDEDMLAYQIEGMESIIGHAE
TMVTALMDRLRAMCDAVMPRKRNTKRKPPVYWWSDSLHQLRTECIKARRQ
AQRSRGQPHHSQCIEVYKAKRTELKNGISAAKANAFKDLIDSVDDDPWGL
AYKVVRKKLNSAGAGSPQDPAALANIVSELFPSQHTLWQPAVDPPASDFP
CITSCEVVEAAKRIRPNKAPGIDGIPGVIVKAAATARPEVFRDTFQQCLL
DGVFPKRWKKMKLVLLLKGKGPANVPRSYRPLCLLDIVGKLFERILYARI
ELITESPTGLQGQQYGFRKGKSTLDALKSVTDAARKALDGNRWLGGSKKY
CAIITLDVKNAFNTARWPIILGAMRNLGVPDYIRGVIGNYFRDRVLWYVT
EDGPRSHQVSAGVPQGSVLGPILWNIMYDGILSISKPRGVELHCFADDVA
ITAVAKTIPELQDISNVAITAAIEWLEKVGLKIAAHKTEVVLLSSRKSVE
CMRVEVKGVEIASAETLKYLGVLIDRRLSFKAHARYASKKAAMTAAALAR
IMPNVGGPRLPARRLLVAVSKATLLYAAPIWSCVSTKKTYLDSARAVSRT
MALRLIRGFRTISDDAAHALSGITPIDLDIKGKYLASEGYTQLEIKEWIR
GVWQTRWQESQRGRWTYKLIPQLTEWADCEHKTVDYHMTQFLTDHGCFRG
YLCRFRHVDTAQCLYCTDAVETAEHILLHCSRFAEERAQLVALAGSPLSP
RGLVAAMMADKIVWDGAHVIIVTMMKRVRKDEMANRNYR
15004-14994: putative Target Site Duplication.
Full sequence of the Waldo-B element (5180 bp):
CCGCGTATTTCGCACGTCTTTTTGCTGCGCTGCGCGAATTTCGTCATATA
CGAGTCCGGTCAGCCGTAAACTGCAACATAAACCTGCAAATCCACTCTGG
GGTTTGCGCCGCGTTTCTGAGCGTCACGGTGTCGTTCCGGAATTCACGAG
TGATCCAGTAAACTGGTAATAAATCAGATCAGCTGCATCAGACAATACAG
CTGATAGCATTGCCAACCTTTCGCAGTCGACGAGCTGGTTAGACTGGCGT
TGCCAGATCAGCGGTGCGATCGTCATTCGGCCGCGCGCTCGTTGGGCGCG
AAATTCGAATTCAAATTCAAATTTGAACTAATTAAGTTGAACAAAAATTT
GAAATATAACCTAAGAACTGAAGCTAGTTCTATTTCCCACGGAGCGCTGG
GGACCAACAAGCCAAAGTCCCCCTTTTATATCTGATGGACAGAACAGGGG
GGGGCAGTGCCCCCGACCCCAACGACCCGTTTAGGAGGAGTGGTAGGCTA
TCGAGATCCCCTATAAGAGGAGTCGGAACCCAAATCCAGGGAGGGGGAGG
TGAGCAAGCAGGCCCCCCGCCGCCGAAAGCTAGCGACTGCAGTGCAGCTG
TGGAGGTGGAGACTCTGGCCACTACAGTCTCCACGACAGCCACAAGCCTG
AAGACAATGGATTTCATCAGCGTGCCGGCCCAAAGGGCGGGAGCGAAGTC
CCCATCAGGATCGCCGCATCGCTCGCCAGAACTGACCACGACGCTCCAGA
ATGAGGACCTGCAGGGCATCCTGGACATGATGAAAGCCAAGATCACCGCC
ATTCTATCCTCGTTCGAGACTAGGCGTCACGTAACCAGCGAGGATAGGGG
CGTGCTAGTGGACCTATCGGCGCTCAATAAGAGAGCGATTGAGTTACAGG
AGGGTATCAACAAGAAGCCCCCACCAAGGAGTACTGCCACACAGACTGAG
GCAGAAAAGACAAAGCGTAGCCAGGTGGCGCCACCCAGACAGGCATTACC
GAATGTGCAGGTCCGCAAAACGGACAACCATCGATCTGCCGCGAAATCCA
CCGGGAAGGCGTTACCCACGACGGCTGACTCCAAACCGGAAAGTTATGCG
TCTGTCGCCAAGGACGCTAACAAGGACGAGGAATGGGCTAAGGTGAAGCC
CAAGCGCCTGCGCAAAAAACCTGAGGCGCTAATCCTGAAAAAAACGGGTG
AGGTTACGTACTCGGATATGCTCCGGAAGATGAAAGCAGAACCGAGTCTG
ACCGAATTCGGCAAGCACGTGCGTAAAATAAGGAGGACGCAACAGGGAGA
ACTACTTCTTGAATTAGAAGGTAAAGCCTCGGAGGTCATCCCCAGCTTTA
AAAATGAGCTAGAAGCGACGCTCAAAGAGATTGCTTCGGTTCGCACGGGC
GCGCATAGGACTGCGCTAATCTGCAGCGGACTAGACGAGACAACGACGGC
TCAGGACCTTCACAATTCCCTGGTCTCCCAATTTCAGGGCATCCGCCTGG
AACCAGAGGATGTAAGAGGCCTTCGCAGGAGGCGTGACGGGACCCAGATA
GCCTCTGTGCTAATGTGCGCGAACGATGCCATTGCGGTCATCAACCGGGG
CGTTGTAACTGTGGGATGGTCGCGTTGCCGCATAGCCCAAGACGTCCGCC
CAATAAGATGCTTCAGATGCCTCGAATTCGGCCACCGAGCTCCCTACTGC
AAGTCAGTCGATCGCTCTGACTGCTGCCTACGGTGCGGCGAGCATGGGCA
TAAGGCAAAGGGCTGCGTAGCCCCACCAAGATGCCTGATCTGCAGCAGTG
ACGTGGACAAGAACCACGCGACGGGTGGTTTTGCATGCCCCACCTACAAA
GCCAACACCAAAGGAGCTAATAGCCGTCAAAATGATGCCAGAAGAAATTA
ACATCATCCAGCTCAACGTTAACCATTGCGCAGCAGCACAGAACCTCCTG
ACTCAAACAGCGAAGGAGCGCCATGCGGACGTAGTGCTTTTGAGTGAACC
ATACCTACCTGGTGTGGGTAACTCAGGAGTGCTACTCGATGAGACAGGCA
AGGCGGCCATTAAATGTACATCCAGGTTATTGGTAGAGGAGTGGGATACC
GTACCAATGCGCGGCATAGCATACGCCAAAATTAGAGGAATCCACTTCTA
CAGCTGCTATGCTCCACCTAGCGACAGTCCCGAGCAATTCGAGGACATGC
TCGAAAAGCTGGTTAACCATGCAAGTGGGCGCAGACCAACAGTCATCGGA
GGTGACCTCAATGCCTGGGCTACAGAATGGGGCAGTCGAATCTCTAACAC
AAGAGGACGAGCAGTGATCGATGCCATGAACCTGCTAGATCTCGTATTGC
TAAACGACGGGTTCAAACCGACGTTCAATAATGACAGAGGTACATCTTTC
ATTGATGTCACTTTTGTTAGCAGAGTTCTAGTGGCTGGCTCGAACTGGAT
GGTCCATGAGGACATAACGCTGAGCGACCACAACCTAATCACGTTCGGTG
CCCGAACAATGAGGCCGTCACCCAAACAGCGAAGATGTGCGCTAGGTCCG
GTATGGGACATTAGGAAACTGGATGAAGACATGCTAGCATACCAGATCGA
GGGCATGGAGTCCATAATTGGACACGCGGAGACCATGGTGACAGCGCTCA
TGGATAGGCTTAGAGCAATGTGTGATGCGGTGATGCCGAGAAAAAGGAAC
ACGAAACGAAAGCCCCCTGTCTACTGGTGGAGTGACTCATTGCACCAGCT
TCGGACGGAATGCATCAAGGCGAGGAGGCAAGCGCAGCGATCTAGAGGGC
AACCGCACCACTCCCAGTGCATTGAGGTATATAAAGCGAAGCGAACTGAG
CTTAAGAACGGCATATCAGCAGCAAAGGCGAATGCTTTTAAGGACCTGAT
TGATAGCGTAGACGACGACCCCTGGGGTCTCGCCTACAAAGTCGTAAGGA
AAAAACTCAATTCCGCTGGTGCAGGATCTCCTCAGGACCCAGCCGCCCTG
GCCAACATCGTGTCGGAACTATTTCCAAGCCAACACACGTTATGGCAACC
CGCTGTTGACCCCCCCGCCTCTGACTTCCCGTGCATCACGTCATGCGAAG
TCGTCGAAGCAGCAAAGAGGATCAGACCGAACAAAGCTCCCGGCATTGAT
GGTATCCCGGGCGTGATCGTCAAAGCAGCCGCCACCGCAAGACCGGAGGT
CTTCAGGGATACATTCCAACAGTGTCTGCTGGACGGAGTCTTCCCCAAGC
GCTGGAAAAAGATGAAGCTGGTCCTTCTGCTGAAAGGCAAGGGGCCCGCA
AATGTTCCACGCAGTTACCGACCGTTGTGTCTGTTGGACATTGTTGGCAA
GCTCTTTGAGCGCATACTGTACGCGCGCATTGAGCTAATCACTGAAAGTC
CTACAGGCCTTCAGGGCCAACAGTATGGCTTCCGAAAGGGTAAAAGTACG
CTCGACGCTCTCAAATCCGTAACAGACGCTGCCAGGAAAGCACTCGACGG
TAACCGATGGTTAGGCGGCAGTAAGAAGTACTGTGCCATCATCACGCTAG
ACGTCAAGAACGCATTCAATACAGCGAGATGGCCCATTATCCTCGGGGCT
ATGCGCAACTTGGGCGTTCCCGACTACATACGAGGCGTAATTGGCAACTA
CTTCAGGGACCGTGTGTTATGGTACGTAACAGAAGATGGTCCAAGAAGCC
ACCAAGTCTCTGCAGGCGTTCCCCAAGGGTCGGTACTAGGACCGATCTTG
TGGAACATTATGTATGATGGAATACTGAGCATTAGCAAGCCCAGAGGTGT
GGAGCTGCACTGCTTCGCCGACGACGTTGCGATAACAGCGGTCGCCAAGA
CAATACCGGAGCTCCAGGACATAAGTAACGTGGCAATCACGGCGGCCATA
GAATGGCTCGAGAAAGTCGGACTTAAAATAGCTGCGCATAAGACCGAAGT
AGTCCTGCTGAGCAGCAGAAAGTCCGTTGAGTGCATGCGTGTAGAAGTCA
AAGGAGTTGAAATCGCCTCAGCAGAAACGTTGAAATACCTTGGTGTTCTA
ATAGACCGAAGGCTCTCGTTCAAGGCTCACGCAAGGTATGCCAGCAAAAA
AGCGGCAATGACAGCAGCAGCCTTGGCGAGAATCATGCCCAACGTGGGAG
GACCCAGACTGCCGGCTAGGAGACTGTTAGTGGCGGTTTCAAAGGCAACG
CTGCTTTATGCTGCGCCTATCTGGAGCTGCGTTTCCACAAAGAAAACCTA
TCTAGATAGTGCCCGCGCAGTATCACGGACAATGGCTCTCAGGCTAATCA
GAGGCTTTAGAACCATATCGGACGACGCAGCGCACGCTCTGTCAGGCATT
ACACCCATTGACCTGGACATAAAGGGCAAATACCTTGCGAGTGAGGGATA
CACTCAATTAGAGATCAAAGAGTGGATTCGAGGAGTATGGCAGACCAGGT
GGCAAGAGTCACAACGGGGACGCTGGACTTACAAACTCATTCCGCAACTA
ACGGAGTGGGCTGATTGCGAGCACAAAACGGTGGACTACCACATGACCCA
GTTCCTCACGGACCATGGCTGTTTTCGAGGGTACCTATGTAGGTTCCGCC
ACGTGGATACAGCCCAGTGCCTTTATTGCACAGACGCAGTGGAAACCGCA
GAGCACATCCTACTGCACTGCTCCAGGTTCGCCGAGGAGAGGGCGCAACT
CGTGGCGCTCGCTGGGTCACCTCTCAGCCCGAGAGGCTTGGTTGCTGCTA
TGATGGCGGACAAAATCGTTTGGGATGGGGCTCACGTGATCATCGTCACC
ATGATGAAGCGTGTCCGTAAGGACGAGATGGCCAATCGGAACTATAGATA
AGGAGTACCCCCGTATGTTGGCGGGGCAAGAACTCTACGACTGGTACGCT
CAGTTGGTCGTAAAAAGGCGCTGCTGTGCACCGCAAAAGAAGATGGTGTG
CAACTTGGCACCACATCCTGCTCACCGATGAAATACCTTGACTGGCAGTC
CCGGTGAGCTTGACAAGGACAGGAGAGAGAGCGGAGGTTTTTGTTTAGTA
CGTAGGCATAAGCCCTCAGCTGAGGGTTATGAATCGTGCATGCCATCCAA
GGACATTAGATGGTATCTTTAGAAGATTTCATTTTCCTGCCGTATATAAT
AATAAAAAAAAAAAAAAAAAAAAAAAAAAA