Dear Dr Wismar,
I am currently curating your paper for FlyBase:
Wismar et al., 2000, Dev. Biol. 226(1): 1--17
Does the EMS allele wocrdg the same allele as the single EMS allele
mentioned in your abstract from 13th EDRC meeting in Crete?
Wismar et al., 1993, Europ. Dros. Res. Conf. 13: Poster I6
Best wishes,
Chihiro
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Subject: woc allele
Dear Chihiro,
The allel woc rgl is not the same as the one in the abstract of 1993 which
was found in a P-element mutagenesis (not EMS allele as you wrote) but does
not contain a P-lac W insertion. This allel is now called woc st1 as
mentioned in materials and methods.
Sincerely
Jasmine
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Subject: Re: woc allele
Dear Jasmine,
Thanks for your reply. So if I understand you correctly, the wocst1
allele is one of the nine woc alleles found 'during an 'enhancer trap'
mutagenesis' as mentioned in the 1993 abstract. Is this right?
In the next sentence 'One is EMS induced', suggests that you also had
at that time another single EMS induced woc allele. Does this still
exist? Have you given it a name? The reason I ask is that in looking
through our current records, up until your recent paper, we only had
one woc allele recorded \- woc1. It seems that this was created by us
in 1993, based on the statement that describes a single unnamed allele
i.e. 'One is EMS induced'. The name was given arbitrarily by us at
the time (we no longer name unnamed alleles in this way), I would like
to change it to a name assigned by you. Do you have one I can used?
Thanks
Chihiro
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Subject: Re: woc allele
Dear Chihiro,
I know it is confusing with the woc alleles.
We have one EMS induced allel called woc rgl (Dev. Bio. 226, 2000) this was
the one mentioned as 'one is EMS induced' in 1993. All the phenotype
described in this abstract deals about the sterile alleles thus I thought
you have been talking about these alleles in your letter. Sorry for this .
The nine alleles from the enhancer trap mutagenesis seem to be just one
allele as all the PlacW insertions have been at different places on the
third chromosome. Separating the mutation (not yet identified on sequence
level) from the PlacW insertions by crossing over all the 'alleles' show
the same phenotype and have white eyes. Thus we suppose that the nine
alleles are just one allele which we called woc st1, but this has to be
confirmed by sequence analysis (on the way).
I hope that now the situation is clear otherwise ask again.
Sincerely
Jasmine