Subject: Celera sequence for Hsp70 genes
I have been cleaning up the heat shock entries in Swiss-prot and TrEMBL
and I found that Celera had only annotated one of the Hsp70 genes.
CG18743 corresponds to Hsp70Aa, it is a better amino acid match to
Hsp70Aa than Hsp70Ab, but if you believe differently please let me
know.
By translating the entire DNA sequence from AE003693 and AE003696 in all
6 frames I found bits and pieces of all 5 genes but both gene clusters
were in regions of really low sequence quality, lots of Xs. I could
translate a complete Hsp70B gene (all three have the same sequence so I
am unsure which it corresponded to) but it involved 3 frameshifts when
the translation should be contained wholly within 1 coding exon.
I am not altogether sure of your strategy regarding any further
sequencing but from reading
http://www.fruitfly.org/sequence/assembly.html it seems the whole genome
will be re-examined, not targetted sites.
Anyway, the point of this message it to request that these two GenBank
entries will have their sequence examined as the Hsp70s really should be
annotated.
Nellie