Subject: Re: FlyBase query
Dear Gillian,
a,b: Yes. Sema2b enhancer, hsp70 promoter, tau-myc coding seq.
c: CaSpeR derivative (white+)
Thanks,
Barry
>Dear Dr. Dickson,
>
>I am curating your paper for FlyBase:
>
>Rajagopalan et al., 2000, Cell 103(7): 1033-1045
>
>and I have a question about the 'Sema2b-tau myc' cell marker you used
>(page 1037).
>
>I would be grateful if you could tell me some details of this
>construct, so that I can record it in FlyBase:
>
>a. what are the promoter sequences driving expression of this marker \-
>are they Sema2b sequences ?
>
>b. what coding sequences are present \- presumably tau sequences and
>also sequences encoding the myc tag ?
>
>c. what vector is the construct in e.g. CaSpeR, Carnegie-20 (so I can
>work out what the marker allele is.)
>
>Any information you give me would be recorded as a personal
>communication to FlyBase, and since this marker is cited as 'K.A. Senti
>B.J. Dickson unpublished results', perhaps the best thing would be to
>have both you and K.A. Senti as authors of this personal communication?
>
>I look forward to hearing from you,
>
>Gillian
>
>--------------------------------------------------------------
>Gillian Millburn.
>
>FlyBase (Cambridge),
>--------------------------------------------------------------
\--------------------------------------------------
Barry Dickson
Research Institute of Molecular Pathology (I.M.P.)
Dr. Bohr-Gasse 7
A-1030 Vienna
Austria
\--------------------------------------------------