Gene or accession: CG14751
Gene annotation error
Genes CG14751 and CG8643 should be merged.
Comments: I've sequenced the entire EST GH19651 and the cDNA encompasses both
these 'genes'. My EST consensus sequence matches the genomic except in two
places a 'g' has been substituted for 'a' both making amino acid changes.
These two 'g's' are present also in sequence posted for an overlapping EST
(GH06282) ..
Subject: Re: <up>Re: FlyBase error report for CG14751 on Wed Jan 24  18:08:42  2001</up>
Dear Gillian,
Here's an update. It appears that the EST GH27921 is a different splice
variant compared to GH19651, and the area that is present in GH19651 that
causes the two coding frames to be out of frame, is removed in GH27921. So in
effect, GH27921 codes for both of those CG's as one protein. SD02665 and
GH06282 appear to have other splice variations as well but I don't have all
the sequence on those. I'll submit the sequence for GH27921 and GH19651 to
genebank in the next month or so since I'm publishing a paper on this gene in
the next few months. Thanks, Audra
..
Subject: update for FlyBase error report for CG14751
Dear Gillian,
..The bottom line now is that EST GH19651 is likely an incompletely processed
transcript containing a small intron that disrupts the reading frame, while
EST GH27921 has that intron spliced out. .. CG14751 and CG8643 .. do indeed
code for one
large C2H2 zinc finger protein from one transcript. I'm sequencing GH27921 in
its entirety to verify the rest of the sequence with what I got for GH19651.
At least now they'll know that CG14751 and CG8643 are one transcript and one
continuous protein (minus the first MQ of CG6843). Once I have the sequence
verified for GH27921 (in a couple of weeks), I'll submit the entire DNA and
protein sequence to Genbank.
Hope that helps, Audra
..
Subject:  Re:update  for FlyBase error report for CG14751
Hi Gillian,
..I have submitted the sequence to
Genbank for the gene regular (rgr) corresponding to the locus that the EST
GH27921 represents. That Genbank \# is AF353512 and is scheduled for release
in June. From my other e-mail account (which handles attachments better) I'll
send you the protein and DNA sequence for GH27921 I submitted to Genbank as
well as the DNA sequence for GH19651 to help Flybase clarify this region.
Those attachments will be in Word. I hope this helps, Audra
..
Here are the sequence files for the regular locus. Audra <<rgr protein
(GH27921).doc>> <<rgr sequence (GH27921).doc>> <<GH19651 DNA
sequence.doc>>
\------------------------------------------------------------------------------
--
<<rgr protein(GH27921).doc>>
MMPLQESPSPSWQLEDYAFFRKCGEITVSPDVQSFGFNCAFCPAICLQFSVFMDHIRVQHTQDVSRRYETEEERCPQTD
LVIMELDCPEIYPPEAPRKCSWEADMDIQLMSLVPSPPAVHIAPLPPPKTTETPEIDLVYVVENPLPPSPPPSADISVD
ELPGSSPQSFDLDPFAVLHEVVTMTPPTPIAPPPGPPTPNPTPTATPAPLPRYETRRVAMQRKLRQSQNEQPKDGAVDE
VAVEEHKGTEPTGQPEKSAMELTPPATPPTPLPPTSPTSSTASCADFQSKSRRELERNHEFVGCLLREYERTEKLWNPR
HPDYKYNAKRSAYGDLAGPLESICHRQLSGAEIFAVLKELRCRYRRELKKVNALGGKYKSRLWYFERMDFLRCVIENRR
AEREAKISNESTESEKSCETDADSCGKSSLYHEVLSFILDAFKRQECLWNPQHYDYTTCCKTELFRDISVQLQEELNYE
LSGEECCNEIQKLRTRYRKELRMVIKHKGLYLPKLWCYDEMEFLQPILQEQIFNKISKKIGVVGSNQKTKFIDASSIRF
DNTEKQLQFVEIYHNCSALWDVDHPDFRSNTYRSQALGQMLDEINTTFHTSYTAERLEKTLFNLRKEFSAQKRKILTES
EDSSSIPLLHAKLAEFLDQNLGPFRCDICSDLVKTCDQYKVHRSAHDGTQPFICTLCGKGFQMPCNLTVHIRRHRRDFP
YSCEQCDKRFATSTEVAIHLRTHTGERPYICDLCGKSFKTWSFFDIHRRRHLNQSTFHCPICAKGFYEKNRFTDHMNSH
WAIRKHLCTVCGKTFTTYGNLKKHTELHLAVKKYKCGTCGKRFAQFASLRWHKKREHSSVGQAGGK
\------------------------------------------------------------------------------
--
<<rgr sequence (GH27921).doc>>
cagcggcgttggttcggaaaagtgcagtctccgcgtcgcgagtgcgccaataacgagaaataacgagaattgaaaaagt
tccttaagaggtggagtccagtaaagcgcagcagcaatgatgccgctgcaggagtcgccgtcgcccagttggcagctgg
aggactacgctttcttccgcaagtgcggggaaatcaccgtctcgccggacgtgcagagcttcggcttcaactgcgcctt
ctgtccggcgatctgcctgcagttctccgtgttcatggaccacatccgggtgcagcacacgcaggacgtgagccggcgc
tacgagactgaggaggagaggtgtccgcaaaccgatctggtcatcatggaactcgactgccccgagatttatccgccgg
aggcgcctaggaaatgcagctgggaggccgacatggacatccagctaatgagcctcgttccgtcgcctcccgcagtgca
catagcacctctaccaccacctaaaacgaccgaaacaccagaaatagatttggtctatgtggttgaaaatccattacca
ccctcgccgccgccctccgcggacatttcggtcgacgagctgccgggaagcagtccgcagtccttcgacctggatccct
ttgccgtgctgcacgaggtagtaacgatgactccgcccactcccattgctccaccaccgggcccgccgacccccaatcc
gactccaactgcgactccggccccattgccgcggtacgagacacgacgcgtggcaatgcagcgcaagcttcgtcagtcg
caaaacgagcaacctaaagatggggcggtggacgaggtcgcggtggaggagcacaagggaacggaacccactggacagc
cggagaagagtgctatggaactaacaccacctgccacgccgcccaccccactgccgcccacctcgcccacgagcagcac
ggcaagctgtgcggatttccaatcgaaaagtcgcagggagctagaacgtaatcacgagttcgttggctgtctgctgagg
gagtacgagcgcacggaaaagctgtggaatccccggcatccggactacaagtacaatgccaagcgcagtgcctacggtg
atctggccggtccgctggagtccatttgccacaggcagctctccggagccgagatcttcgctgtgctaaaggagttgag
gtgcagataccgccgcgagctgaaaaaggtgaacgccctgggtggaaagtacaagtcgcgtctgtggtactttgagagg
atggactttctgcggtgtgtcatcgaaaacaggcgagccgaaagggaagccaagatttccaatgagagcacagagagtg
aaaagtcgtgcgaaacggacgctgattcctgcggcaagtcatctttgtaccacgaggttctgagtttcattctggatgc
cttcaagaggcaggaatgcctgtggaatccgcagcactacgactacaccacatgctgtaagacggaactgtttcgcgac
atctccgtccagttgcaggaggagctcaactatgagctgagcggcgaggaatgctgcaacgagatccaaaaacttagga
cacgttaccgcaaggagctgcgcatggttattaagcacaagggattgtacctacccaagttatggtgttacgatgaaat
ggagttcctgcagcccatactgcaggagcagatcttcaacaagatcagcaagaaaatcggagtggtgggaagcaaccag
aagaccaagttcatcgatgccagctcaattcgttttgacaatactgagaaacaactgcagtttgtagagatttaccaca
actactcagctctgtgggatgttgaccatcccgacttccgatcgaatacgtatcgtagtcaggctttgggtcaaatgtt
ggatgagataaacacaacctttcacacttcctacactgcggagcagttggaaaagaccttgttcaatctgcgcaaagaa
ttctccgcccagaaacggaagatacttacggagtccgaagactccagcagcattccgctgctgcatgccaaactggcag
agttcctagaccaaaatcttggtccttttcgttgcgatatatgctcggacctggtcaagacctgcgatcagtacaaggt
gcatcgatccgcacacgatggcacccaacccttcatctgcactc
\------------------------------------------------------------------------------
--
<<GH19651 DNAsequence.doc>>
gccggttgtttggatcgcagattgaatcggaaagccagcggcgttggttcggaaaagtgcagtctccgcgtcgcgagtg
cgccaataacgagaaataacgagaattgaaaaagttccttaagaggtggagtccagtaaagcgcagcagcaatgatgcc
gctgcaggagtcgccgtcgcccagttggcagctggaggactacgctttcttccgcaagtgcggggaaatcaccgtctcg
ccggacgtgcagagcttcggcttcaactgcgccttctgtccggcgatctgcctgcagttctccgtgttcatggaccaca
tccgggtgcagcacacgcaggacgtgagccggcgctacgagactgaggaggagaggtgtccgcaaaccgatctggtcat
catggaactcgactgccccgagatttatccgccggaggcgcctaggaaatgcagctgggaggccgacatggacatccag
ctaatgagcctcgttccgtcgcctcccgcagtgcacatagcacctctaccaccacctaaaacgaccgaaacaccagaaa
tagatttggtctatgtggttgaaaatccattaccaccctcgccgccgccctccgcggacatttcggtcgacgagctgcc
gggaagcagtccgcagtccttcgacctggatccctttgccgtgctgcacgaggtagtaacgatgactccgcccactccc
attgctccaccaccgggcccgccgacccccaatccgactccaactgcgactccggccccattgccgcggtacgagacac
gacgcgtgcgccagcgattgtgtacgatttccagcaccacgataaaccccggacaagatcgtctggctgggcagcgctt
atctcgcagagagcccaacactggcaacgttacaaatttatttatttatgcgaaatatttatacttactctatactcta
tgcaggcaatgcagcgcaagcttcgtcagtcgcaaaacgagcaacctaaagatggggcggtggacgaggtcgcggtgga
ggagcacaagggaacggaacccactggacagccggagaagagtgctatggaactaacaccacctgccacgccgcccacc
ccactgccgcccacctcgcccacgagcagcacggcaagctgtgcggatttccaatcgaaaagtcgcagggagctagaac
gtaatcacgagttcgttggctgtctgctgagggagtacgagcgcacggaaaagctgtggaatccccggcatccggacta
caagtacaatgccaagcgcagtgcctacggtgatctggccggtccgctggagtccatttgccacaggcagctctccgga
gccgagatcttcgctgtgctaaaggagttgaggtgcagataccgccgcgagctgaaaaaggtgaacgccctgggtggaa
agtacaagtcgcgtctgtggtactttgagaggatggactttctgcggtgtgtcatcgaaaacaggcgagccgaaaggga
agccaagatttccaatgagagcacagagagtgaaaagtcgtgcgaaacggacgctgattcctgcggcaagtcatctttg
taccacgaggttctgagtttcattctggatgccttcaagaggcaggaatgcctgtggaatccgcagcactacgactaca
ccacatgctgtaagacggaactgtttcgcgacatctccgtccagttgcaggaggagctcaactatgagctgagcggcga
ggaatgctgcaacgagatccaaaaacttaggacacgttaccgcaaggagctgcgcatggttattaagcacaagggattg
tacctacccaagttatggtgttacgatgaaatggagttcctgcagcccatactgcaggagcagatcttcaacaagatca
gcaagaaaatcggagtggtgggaagcaaccagaagaccaagttcatcgatgccagctcaattcgttttgacaatactga
gaaacaactgcagtttgtagagatttaccacaactgctcagctctgtgggatgttgaccatcccgacttccgatcgaat
acgtatcgtagtcaggctttgggtcaaatgttggatgagataaacacaacctttcacacttcctacactgcggagcggt
tggaaaagaccttgttcaatctgcgcaaagaattctccgcccag
\------------------------------------------------------------------------------
--