Gene or accession: CG8674
Release: 1
Missed gene
Comments: CG8674 is similar to yeast gene YJL180C (gene name ATP12).
Smith-Waterman searching revealed that these two genes are each other's best
match in the fly and yeast proteomes. They share a 23% identity in an
overlapping region extending the length of each sequence. PSI-BLAST with
either YJL180C (BL62 or BL45) or CG8674 (BL45) identify the same set of six
sequences from S. cerevisiae, S. pombe, C. elegans, D. melanogaster, A.
thaliana. The SW alignment that triggered this investigation is below:
(BLOSUM50 \-12 gap initiation, \-2 gap extension)
> ORFP:YJL180C  ATP12, Chr X from 87581-88558, reverse complement
(325 aa)
s-w opt: 146
Smith-Waterman score: 146; 22.930% identity (27.799% ungapped) in 314 aa
overlap (3- 262:8-320 )
10 20 30 40 50
60
CG8674
MNGKYVVSAIRALRLTNF----SQCKGAASSFT------VRHYASPPKRFYKKTSVLSG-DSG-YEVVLDHRKLK
 : ..:..:: :.: : .: :. ... . . .. ..:..:.:. ..:
. :: : .:
 ORFP:Y 
MLPSLRKGCFIVNSIR-LKLPRFYSLNAQPLGTDNTIENNTPTETNRLSKTSQKFWEKVSLNRDVEKGKIALQLDGRTIK
10 20 30 40 50 60 70
70 80 90 100 110
120
CG8674
TPKGTPFIVRSEP--LAIAVATEFDA-QKENIERSRMHLSALCFTAID---------NPNHLSKL----DMV-NYLLNFI
 :: :. .:: . :: . :... .. .:. . :..: :: .:. ..:.
 :.. : :: ..
 ORFP:Y 
TPLGNGIIVDNAKSLLAYLLKLEWSSLSSLSIKTHSLPLTSLVARCIDLQMTNEPGCDPQLVAKIGGNSDVIKNQLLRYL
80 90 100 110 120 130 140 150
130 140 150 160 170 180
190
CG8674
ATDTVL-FQYDDEKD--LQDLQVNEWDPVIAW---FNQRYDTNLQKTMNITPPQV-----SEQDKM--NVAKHFQSY---
 :::.: :. .: . :.. : . . :.: : . .... . ..: .. ..:. .
 :.::...:
 ORFP:Y 
DTDTLLVFSPMNEFEGRLRNAQNELYIPIIKGMEEFLRNFSSESNIRLQILDADIHGLRGNQQSDIVKNAAKKYMSSLSP
160 170 180 190 200 210 220 230
200 210 220 230 240 250
260
CG8674
\--------SLETLHGFIFAVDTLKSIVLACAVIEQMLT-VEKAVALARLEEEYQLKFWGRVEWAHDLSQQELQARLAA
AV
.. : ..:: .: :.. . .: . : ... : : :: .:.. ::.::
.::....... .. .:.
 ORFP:Y 
WDLAILEKTVLTTKSFICGVLLLENKKDTANLIPALKTDMDNIVRAATLETIFQVEKWGEVEDTHDVDKRDIRRKIHTAA
240 250 260 270 280 290 300 310
270
CG8674 LFVHLNCSENLVKQKIIL
.
 ORFP:Y  IAAFKQ
320