Gene or accession: CG12348
Release: 2
DNA error at position 98153. Upstream nucleotides: tttatttcttcactaaatacc
Insertion of length 1: c.
Corrected sequence: tttatttcttcactaaatacgtctaaa.
Comments: I have for SWISS-PROT looked at the Shaker sequence. The insertion
is found by
comparing to M17211. The CDS (and mRNA) is, furthermore, badly predicted.
I here show a BLAST of M17211 against the genome: Note that (at least) 5
other isoforms exist
>emb|AE003507|AE003507 <up>Drosophila melanogaster</up>Drosophila melanogaster
genomic scaffold 142000013386053 section 24 of 30,
complete sequence.
Length = 297308
Score = 278 bits (140), Expect = 2e-72
Identities = 140/140 (100%)
Strand = Plus / Minus
Query: 8 ggagtttctatccagacttcaatatttttttacctcgctcaaaaccccccactcgcactt 67
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 135947 ggagtttctatccagacttcaatatttttttacctcgctcaaaaccccccactcgcactt
135888
Query: 68 taaataataaaaaaaagcaggtggtgcgtgccgcgtagccgcgcgtgattcttgttgttg 127
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 135887 taaataataaaaaaaagcaggtggtgcgtgccgcgtagccgcgcgtgattcttgttgttg
135828
Query: 128 ttttttttttttcggtgaat 147
||||||||||||||||||||
Sbjct: 135827 ttttttttttttcggtgaat 135808
Score = 878 bits (443), Expect = 0.0
Identities = 443/443 (100%)
Strand = Plus / Minus
Query: 148 ctcttgtaaccatgtaccaaagttctttgccgcgaaaactaaaatgaaaacgaaagtgaa 207
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 122264 ctcttgtaaccatgtaccaaagttctttgccgcgaaaactaaaatgaaaacgaaagtgaa
122205
Query: 208 aatgagcgaatggcagccgcggccacagcaatcgatccatgacacaaccagtgacaagca 267
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 122204 aatgagcgaatggcagccgcggccacagcaatcgatccatgacacaaccagtgacaagca
122145
Query: 268 gtcccccagtgaaaccgcatccgcatccgagtccgataccgataaagattctgaatcgga 327
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 122144 gtcccccagtgaaaccgcatccgcatccgagtccgataccgataaagattctgaatcgga
122085
Query: 328 gtgagtgccgcgtccgagagcgttccctgtccacgtccaccatcggcggagcaggtgtgc 387
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 122084 gtgagtgccgcgtccgagagcgttccctgtccacgtccaccatcggcggagcaggtgtgc
122025
Query: 388 ctgaggcccacctggtggcatggccgccgttgccggcctctatggccttggggaggatcg 447
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 122024 ctgaggcccacctggtggcatggccgccgttgccggcctctatggccttggggaggatcg
121965
Query: 448 ccagcaccgcaagaagcagcagcaacagcagcagcaccagaaggagcagctcgagcagaa 507
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121964 ccagcaccgcaagaagcagcagcaacagcagcagcaccagaaggagcagctcgagcagaa
121905
Query: 508 ggaggagcaaaagaagatcgccgagcggaagctgcagctgcgggagcagcagctccagcg 567
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121904 ggaggagcaaaagaagatcgccgagcggaagctgcagctgcgggagcagcagctccagcg
121845
Query: 568 caactccctcgatggttacgggt 590
|||||||||||||||||||||||
Sbjct: 121844 caactccctcgatggttacgggt 121822
Score = 252 bits (127), Expect = 1e-64
Identities = 127/127 (100%)
Strand = Plus / Minus
Query: 588 ggtctttgcccaaattgagcagtcaagacgaagaagggggggctggtcatggctttggtg 647
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 98766 ggtctttgcccaaattgagcagtcaagacgaagaagggggggctggtcatggctttggtg 98707
Query: 648 gcggaccgcaacactttgaacccattcctcacgatcatgatttctgcgaaagagtcgtta 707
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 98706 gcggaccgcaacactttgaacccattcctcacgatcatgatttctgcgaaagagtcgtta 98647
Query: 708 taaatgt 714
|||||||
Sbjct: 98646 taaatgt 98640
Score = 339 bits (171), Expect = 7e-91
Identities = 171/171 (100%)
Strand = Plus / Minus
Query: 713 gtaagcggattaaggtttgagacacaactacgtacgttaaatcaattcccggacacgctg 772
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 98575 gtaagcggattaaggtttgagacacaactacgtacgttaaatcaattcccggacacgctg 98516
Query: 773 cttggggatccagctcggagattacggtactttgacccgcttagaaatgaatattttttt 832
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 98515 cttggggatccagctcggagattacggtactttgacccgcttagaaatgaatattttttt 98456
Query: 833 gaccgtagtcgaccgagcttcgatgcgattttatactattatcagagtggt 883
|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 98455 gaccgtagtcgaccgagcttcgatgcgattttatactattatcagagtggt 98405
Score = 180 bits (91), Expect = 4e-43
Identities = 98/99 (98%), Gaps = 1/99 (1%)
Strand = Plus / Minus
Query: 881 ggtggccgactacggagaccggtcaatgtccctttagacg-tatttagtgaagaaataaa 939
|||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct: 98193 ggtggccgactacggagaccggtcaatgtccctttagacggtatttagtgaagaaataaa 98134
\* Insertion in genome
Query: 940 attttatgaattaggtgatcaagcaattaataaattcag 978
|||||||||||||||||||||||||||||||||||||||
Sbjct: 98133 attttatgaattaggtgatcaagcaattaataaattcag 98095
Score = 400 bits (202), Expect = e-109
Identities = 202/202 (100%)
Strand = Plus / Minus
Query: 976 cagagaggatgaaggctttattaaagaggaagaaagaccattaccggataatgagaaaca 1035
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 97031 cagagaggatgaaggctttattaaagaggaagaaagaccattaccggataatgagaaaca 96972
Query: 1036 gagaaaagtctggctgctcttcgagtatccagaaagttcgcaagccgccagagttgtagc 1095
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 96971 gagaaaagtctggctgctcttcgagtatccagaaagttcgcaagccgccagagttgtagc 96912
Query: 1096 cataattagtgtatttgttatattgctatcaattgttatattttgtctagaaacattacc 1155
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 96911 cataattagtgtatttgttatattgctatcaattgttatattttgtctagaaacattacc 96852
Query: 1156 cgaatttaagcattacaaggtg 1177
||||||||||||||||||||||
Sbjct: 96851 cgaatttaagcattacaaggtg 96830
Score = 250 bits (126), Expect = 5e-64
Identities = 126/126 (100%)
Strand = Plus / Minus
Query: 1173 aggtgttcaatacaacaacaaatggcacaaaaatcgaggaagacgaggtgcctgacatca 1232
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 96733 aggtgttcaatacaacaacaaatggcacaaaaatcgaggaagacgaggtgcctgacatca 96674
Query: 1233 cagatcctttcttccttatagaaacgttatgtattatttggtttacatttgaactaactg 1292
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 96673 cagatcctttcttccttatagaaacgttatgtattatttggtttacatttgaactaactg 96614
Query: 1293 tcaggt 1298
||||||
Sbjct: 96613 tcaggt 96608
Score = 315 bits (159), Expect = 9e-84
Identities = 159/159 (100%)
Strand = Plus / Minus
Query: 1293 tcaggttcctcgcatgtccgaacaaattaaatttctgcagggatgtcatgaatgttatcg 1352
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 96186 tcaggttcctcgcatgtccgaacaaattaaatttctgcagggatgtcatgaatgttatcg 96127
Query: 1353 acataatcgccatcattccgtactttataacactagcgactgtcgttgccgaagaggagg 1412
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 96126 acataatcgccatcattccgtactttataacactagcgactgtcgttgccgaagaggagg 96067
Query: 1413 atacgttaaatcttccaaaagcgccagtcagtccacagg 1451
|||||||||||||||||||||||||||||||||||||||
Sbjct: 96066 atacgttaaatcttccaaaagcgccagtcagtccacagg 96028
Score = 347 bits (175), Expect = 3e-93
Identities = 175/175 (100%)
Strand = Plus / Minus
Query: 1449 aggacaagtcatcgaatcaggctatgtccttggcaatattacgagtgatacgattagttc 1508
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 95510 aggacaagtcatcgaatcaggctatgtccttggcaatattacgagtgatacgattagttc 95451
Query: 1509 gagtatttcgaatatttaagttatctaggcattcgaagggtttacaaatattaggacgaa 1568
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 95450 gagtatttcgaatatttaagttatctaggcattcgaagggtttacaaatattaggacgaa 95391
Query: 1569 ctctgaaagcctcaatgcgggaattaggtttacttatatttttcttatttatagg 1623
|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 95390 ctctgaaagcctcaatgcgggaattaggtttacttatatttttcttatttatagg 95336
Score = 266 bits (134), Expect = 8e-69
Identities = 134/134 (100%)
Strand = Plus / Minus
Query: 1620 taggcgtcgtactcttctcatcggcggtttattttgcggaagctggaagcgaaaattcct 1679
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 90767 taggcgtcgtactcttctcatcggcggtttattttgcggaagctggaagcgaaaattcct 90708
Query: 1680 tcttcaagtccatacccgatgcattttggtgggcggtcgttaccatgaccaccgttggat 1739
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 90707 tcttcaagtccatacccgatgcattttggtgggcggtcgttaccatgaccaccgttggat 90648
Query: 1740 atggtgacatgacg 1753
||||||||||||||
Sbjct: 90647 atggtgacatgacg 90634
Score = 359 bits (181), Expect = 7e-97
Identities = 190/193 (98%)
Strand = Plus / Minus
Query: 1753 gcccgtcggcttctggggcaaaattgtcggctctttgtgcgtggtcgctggtgtgctgac 1812
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
Sbjct: 76205 gcccgtcggcttctggggcaaaattgtcggctctttgtgcgtgatcgctggtgtgctgac 76146
Query: 1813 aatcgcactgccggtaccggttatcgtcagtaatttcaattacttctatcaccgcgaagc 1872
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct: 76145 aatcgcactgccggtaccggttatcgtcagtaatttcaattacttctatcaccgcgaaac 76086
Query: 1873 ggatcgggaggagatgcagagccaaaatttcaaccacgttacaagttgttcatatttacc 1932
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 76085 ggatcaggaggagatgcagagccaaaatttcaaccacgttacaagttgttcatatttacc 76026
Query: 1933 tggtgcactaggt 1945
|||||||||||||
Sbjct: 76025 tggtgcactaggt 76013
Score = 642 bits (324), Expect = 0.0
Identities = 324/324 (100%)
Strand = Plus / Minus
Query: 1941 taggtcaacatttgaagaaatcctcactctccgaatcgtcgtcggacataatggatttgg 2000
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 75869 taggtcaacatttgaagaaatcctcactctccgaatcgtcgtcggacataatggatttgg 75810
Query: 2001 atgatggcattgatgcaaccacgccaggtctgactgatcacacgggccgccacatggtgc 2060
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 75809 atgatggcattgatgcaaccacgccaggtctgactgatcacacgggccgccacatggtgc 75750
Query: 2061 cgtttctcaggacacagcagtcattcgagaagcagcagctccagcttcagctgcagctgc 2120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 75749 cgtttctcaggacacagcagtcattcgagaagcagcagctccagcttcagctgcagctgc 75690
Query: 2121 agcagcagtcgcagtcgccgcacggccaacagatgacgcagcagcagcagctgggccaga 2180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 75689 agcagcagtcgcagtcgccgcacggccaacagatgacgcagcagcagcagctgggccaga 75630
Query: 2181 acggcctaaggagcacaaatagtttacagttaaggcataataacgcgatggccgtcagta 2240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 75629 acggcctaaggagcacaaatagtttacagttaaggcataataacgcgatggccgtcagta 75570
Query: 2241 ttgagaccgacgtctgactactag 2264
||||||||||||||||||||||||
Sbjct: 75569 ttgagaccgacgtctgactactag 75546
Score = 1306 bits (659), Expect = 0.0
Identities = 659/659 (100%)
Strand = Plus / Minus
Query: 2263 agtcaaacaaatggaaaatggacgaaatttgcgcagtgaaatgctacgttggatgccaga 2322
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 75134 agtcaaacaaatggaaaatggacgaaatttgcgcagtgaaatgctacgttggatgccaga 75075
Query: 2323 aacgtcatcaaaagcagtctaatttagaattttattaataaatacaattaaaatataatt 2382
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 75074 aacgtcatcaaaagcagtctaatttagaattttattaataaatacaattaaaatataatt 75015
Query: 2383 ataataattagtaagcaacgtagttgtaaattaaacagcaaatgtacacagacacaacac 2442
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 75014 ataataattagtaagcaacgtagttgtaaattaaacagcaaatgtacacagacacaacac 74955
Query: 2443 acacacagacacagtgccagttcactcagcttgaattagagtatttgtagacaccaaaaa 2502
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 74954 acacacagacacagtgccagttcactcagcttgaattagagtatttgtagacaccaaaaa 74895
Query: 2503 gagtcaaatatggactggccttctatagggatttccttgtttctcctttcattttccttc 2562
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 74894 gagtcaaatatggactggccttctatagggatttccttgtttctcctttcattttccttc 74835
Query: 2563 tggtaatctacacaccgaaaacacttacacacacacgtccacacacactcaaagtaaaaa 2622
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 74834 tggtaatctacacaccgaaaacacttacacacacacgtccacacacactcaaagtaaaaa 74775
Query: 2623 ctctacttgatacctatgttcaaatttagcaattaacaactaacaatcgttaacaacaac 2682
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 74774 ctctacttgatacctatgttcaaatttagcaattaacaactaacaatcgttaacaacaac 74715
Query: 2683 aaaacaaaacatataaaaccaaaaaacgagagaaaaaaaaaaacaaacaaaaccaaaatc 2742
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 74714 aaaacaaaacatataaaaccaaaaaacgagagaaaaaaaaaaacaaacaaaaccaaaatc 74655
Query: 2743 taattatcttagtagactaatctaattggagtttcttcctttctttagaagctagcaaaa 2802
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 74654 taattatcttagtagactaatctaattggagtttcttcctttctttagaagctagcaaaa 74595
Query: 2803 caaaaacaaagaacaacaacaaccagacaaaaacaaacatacaatatctgctaattttat 2862
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 74594 caaaaacaaagaacaacaacaaccagacaaaaacaaacatacaatatctgctaattttat 74535
Query: 2863 tttcatctttaaattatgctctattattaaatattagtcagaatattagtaaaacaaac 2921
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 74534 tttcatctttaaattatgctctattattaaatattagtcagaatattagtaaaacaaac 74476