Subject: l(2)k08316 not mapped in GeneSeen
The P element insertion line flanking sequence maps unambiguously
(100% match 388 nt) to a site in the px gene, but it is not mapped on
GeneSeen in this region. According to Spradling et al. 1999, this
strain is part of the primary collection. I think there is a stock
for it in the Bloomington stock center.
\------------------------------------
Robert W. Levis, Ph.D.
Carnegie Institution of Washington
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Subject: Re: l(2)k08316 not mapped in GeneSeen (fwd)
Hi Bob, Guochun, and Lynn,
I'm glad we've sorted out that the sequence from the line is chimeric. ...
Bob, I tried BLASTing at NCBI also and have no clue what that DNA might be
from!
Best wishes,
sima
> Sima & Guochun,
>
> Sorry for the misinformation about the % identity in the alignment
> with genommic. I must have been looking at the alignment of the
> insertion sequence with itself rather than the genomic sequence. Out
> of curiosity, I tried BLASTing just the sequence that doesn't align
> well with genomic (312-388) against all Drosophila sequences and got
> no good hits. I wonder where it comes from?
>
> ...Bob
>
> >Hi Sima,
> >This element l.2.k08316 only has 311/388 bases mapped to genomic sequence
> >both release 1 and 2. The automatic analysis thought it's a chimeric and
> >left it out. The insertion site should be 2R, 17451994, cytological
> >location 58E5.
> >
> >-guochun
> >
> >On Tue, 20 Feb 2001, Sima Misra wrote:
> >
> >>
> >> Hey Guochun,
> >>
> >> could you look this one up please?
> >>
> >> Many thanks,
> >> sima
> >>
> >> \---------- Forwarded message \----------
> >> Subject: l(2)k08316 not mapped in GeneSeen
> >>
> >> The P element insertion line flanking sequence maps unambiguously
> >> (100% match 388 nt) to a site in the px gene, but it is not mapped on
> >> GeneSeen in this region. According to Spradling et al. 1999, this
> >> strain is part of the primary collection. I think there is a stock
> >> for it in the Bloomington stock center.
> >> \--
> >> \------------------------------------
> >> Robert W. Levis, Ph.D.
> >> Carnegie Institution of Washington
> >>
> >>
>