Gene or accession: CG13411
Release: 1
Gene annotation error
Gene CG13411 should be split.
Comments: Clyne et al., 2000, Science 287(5459): 1830--1834 (FBrf0126794)
describe 2
gustatory receptor genes, GR93F.2 (FBgn0041228) and GR93F.3 (FBgn0041227)
whose sequences match different regions of CG13411 (FBgn0038907), suggesting
either that CG13411 needs splitting into 2 separate genes, or (given that the
sequences of GR93F.2 and GR93F.3 in FBrf0126794 are said to be partial) that
there is only one gene and GR93F.2 and GR93F.3 need merging,
Sequences of GR93F.2 and GR93F.3 (worked out from coordinates given in
References and Notes of FBrf0126794) and alignments to CG13411 follow.
Subject: CG13411 alignments
sequence of GR93F.2 (from FBrf0126794):
(coordinates in sequence header are relative to the clone sequence as
of 5 January 2000)
(partial sequence)
>GR93F.2,AC012892,2781-2650
ctgtactagaaagactagataggtcaccattgccgagagaaagaacagggtcagttcgttggagacctcaaagaggccc
aaaggaaacactcgcagctcctggtgttgaaggcggcccaggaaaagctccag
BLASTN against CGs:
Query= GR93F.2,AC012892,2781-2650, 132 bases, E2A12F30 checksum.
(132 letters)
Database: na_gadfly.dros
14,217 sequences; 24,499,473 total letters.
Searching....10....20....30....40....50....60....70....80....90....100% done
Smallest
Sum
High Probability
Sequences producing High-scoring Segment Pairs: Score P(N) N
CG13411|FBgn0038907|CT32767|FBan0013411  last_updated:000321  660 9.8e-25 1
CG13714|FBgn0040701|CT33180|FBan0013714  last_updated:000321  114 0.93 1
>CG13411|FBgn0038907|CT32767|FBan0013411  last_updated:000321 
Length = 1995
Minus Strand HSPs:
Score = 660 (99.0 bits), Expect = 9.8e-25, P = 9.8e-25
Identities = 132/132 (100%), Positives = 132/132 (100%), Strand = Minus / Plus
Query: 132 CTGGAGCTTTTCCTGGGCCGCCTTCAACACCAGGAGCTGCGAGTGTTTCCTTTGGGCCTC 73
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1837 CTGGAGCTTTTCCTGGGCCGCCTTCAACACCAGGAGCTGCGAGTGTTTCCTTTGGGCCTC 1896
Query: 72 TTTGAGGTCTCCAACGAACTGACCCTGTTCTTTCTCTCGGCAATGGTGACCTATCTAGTC 13
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1897 TTTGAGGTCTCCAACGAACTGACCCTGTTCTTTCTCTCGGCAATGGTGACCTATCTAGTC 1956
Query: 12 TTTCTAGTACAG 1
||||||||||||
Sbjct: 1957 TTTCTAGTACAG 1968
sequence of GR93F.3 (from FBrf0126794):
(coordinates in sequence header are relative to the clone sequence as
of 5 January 2000)
(partial sequence)
>GR93F.3,AC012892,4271-4143
ctgaaccacataggtaaagtaggtgatcatacttgacagaaacagcaagatgacctcgttggatacttcgaatagtccc
aagggtctaacgcgaaactcgaagaagttcagacggcttaagaacatttc
BLASTN against CGs:
Query= GR93F.3,AC012892,4271-4143, 129 bases, C9A98456 checksum.
(129 letters)
Database: na_gadfly.dros
14,217 sequences; 24,499,473 total letters.
Searching....10....20....30....40....50....60....70....80....90....100% done
Smallest
Sum
High Probability
Sequences producing High-scoring Segment Pairs: Score P(N) N
CG13411|FBgn0038907|CT32767|FBan0013411  last_updated:000321  640 8.1e-24 1
>CG13411|FBgn0038907|CT32767|FBan0013411  last_updated:000321 
Minus Strand HSPs:
Score = 640 (96.0 bits), Expect = 8.1e-24, P = 8.1e-24
Identities = 128/128 (100%), Positives = 128/128 (100%), Strand = Minus / Plus
Query: 129 GAAATGTTCTTAAGCCGTCTGAACTTCTTCGAGTTTCGCGTTAGACCCTTGGGACTATTC 70
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1039 GAAATGTTCTTAAGCCGTCTGAACTTCTTCGAGTTTCGCGTTAGACCCTTGGGACTATTC 1098
Query: 69 GAAGTATCCAACGAGGTCATCTTGCTGTTTCTGTCAAGTATGATCACCTACTTTACCTAT 10
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1099 GAAGTATCCAACGAGGTCATCTTGCTGTTTCTGTCAAGTATGATCACCTACTTTACCTAT 1158
Query: 9 GTGGTTCA 2
||||||||
Sbjct: 1159 GTGGTTCA 1166