Subject: Helping FlyBase: P17en1 and P17en43 Dear Judith, I have been curating this qvr paper for FlyBase \*x FBrf0130151 == Wang et al., 2000, J. Neurosci. 20(16): 5958--5964 In it the authors mention (as coming from you) 'P17en1' and 'P17en43' which are evidently insertions into the genome, near qvr. We have no record of these insertions in FlyBase. Could you tell me whether they have been published anywhere? If not, perhaps you could be specific about which P element construct is inserted in these two lines and where they are inserted into the genome? Then I can make proper insertion records for them in FlyBase. Many thanks for your help, With best wishes, Rachel. Subject: Re: Helping FlyBase: P17en1 and P17en43 Rachel, Both 17en1 and 17en43 were insertions of Pen1 into chromosomal subdivision 48A. You can use Kassis et al., 1992, PNAS 89, 1919-1923 for a reference. Judy Subject: Re: Helping FlyBase: P17en1 and P17en43 Thanks Judith, >Both 17en1 and 17en43 were insertions of Pen1 into chromosomal >subdivision 48A. You can use Kassis et al., 1992, PNAS 89, 1919--1923 for a >reference. 17en1 and 17en43 do not apppear to be mentioned explicitly in Kassis et al., 1992. We have record for P{en1}48A from that publication but since we have a synonym of 1-en-14 for that it seems to be a different insertion. We also have a record for something called P{en1}2-en-17 but the reference for that is Perrimon et al., 1991 Generating lineage-specific markers to study Drosophila development. Dev. Genet. 12: 238--252 <up>FBrf0053745</up> so it is probably something COMPLETELY different. I will curate this mail from you as a personal communication to FlyBase, to serve as the reference for these insertions. How about P{en1}17-1 and P{en1}17-43 as insertion identifiers? Thanks again for your help. best wishes, Rachel.