Subject: Helping FlyBase: ADRC-10547
Dear Bruce,
We are currently curating the abstracts for the upcoming 42nd
(Washington, D.C.) Annual Drosophila Research Conference, for FlyBase.
I am writing in connection with your abstract:
'Drosophila as a Model System to Study Disease Associated with Defects in
Peroxisomal Assembly and Function.'
You mention a gene that is new to FlyBase, Dhap-at. Do you know which
of the Genome Project CG annotations your gene corresponds to? All the
CGs have corresponding gene records in FlyBase already and we don't
like to make duplicate records for what is actually the same gene
unless we can't avoid it. If your gene does not correspond to a CG
then perhaps you could tell me its map location, as this is valuable
information for the genome annotation project.
Thank you for your help,
with best wishes,
Rachel.
Subject: Re: Helping FlyBase: ADRC-10547
Hi Rachel,
I apologize for the delay in getting back to you. The cDNAs for
DHAP-AT (Dihydroxyacetone phosphate acyl transferase, aka DAP-AT) we
have correspond to CG4625. A while ago, we had submitted sequence for
this cDNA to Genbank thinking it encoded a closely related enzyme,
glycerol 3-phosphate acyltransferase (GPAT). This is almost certainly
wrong, and we need to correct the Genbank submission (I will do this
shortly). The reason we think we were wrong is that subsequent to the
submission, we noticed that there was a targeting peptide (AKL) at the
C-terminus of the predicted protein that directed it to the
peroxisome. Based on this prediction of the subcellular location of
the enzyme, and other evidence of homology with DHAP-ATs from other
species, we now think that this transcript now encodes Drosophila
DHAP-AT and not GPAT. We do not have direct evidence (e.g., expressing
the full-length protein and testing its enzymatic activity). We have
also identified (in the genome project database) another transcript
that shows considerable sequence similarity to GPATs in other species,
and is probably the 'real Drosophila GPAT'. We are assessing the
enzymatic activity of the expressed protein at this time. I am
attaching a copy of part of a simple search that might be helpful in
clarifying what I have tried to describe above. Let me know if you
have questions or would like additional information. I am happy to
share it.
Bruce
<up>Attached file was copy of GadFly page of CG4625</up>