Subject: Helping FlyBase: Fus-6
Dear Christos,
I am writing to you about a/two enhancer trap insertion(s) in FlyBase.
We have two distinct records at the moment, for
FBti0005754 ==== P{lacW}B9-3-52
FBti0010411 ==== P{lacZ}hdcFus-6
The question is, are these the same thing or not?
The relevent references are:
FBrf0122647 ==== Jan, 1990.8.1, personal communication to FlyBase
FBrf0057603 ==== Hartenstein and Jan, 1992, Roux Arch. dev. Biol. 201(4): 1=
94--220
FBrf0090781 ==== Samakovlis et al., 1996, Development 122(11): 3531--3536
FBrf0101899 ==== Steneberg et al., 1998, Genes Dev. 12(7): 956--967
FBrf0101899 states unambiguously (page 965, 3rd last line) that 'The
Fus-6, B9-3-52 enhancer trap allele was generated in the lab of Y.N.
Jan' and references FBrf0057603.
FBrf0057603 does not address the viability of B9-3-52
FBrf0122647 says B9-3-52 is viable
FBrf0101899 says Fus-6 is lethal
FBrf0057603 doesn't mention trachea though says they looked at stages 16/17
FBrf0101899 sees tracheal expression at stage 14
FBrf0090781 suggests tracheal expression at stage 15
FBrf0101899 says Fus-6 is at 99F
FBrf0090781 says Fus-6 is at 99D
help!
we are not sure how best to resolve this confusion \- I think you are
the best person to help us out. Can you throw any light on this for
us? We would be most grateful for any help you can give.
With best regards,
Rachel.
Subject: Helping FlyBase: Fus-6
Dear Rachel,
I'm answering in Christos place on the message you sent the 1rst of March.
P(lacW)B9-3-52 and P(lacZ)hdc(Fus-6) is the same strain and it is a lethal
P-element insertion.
Ref. FBrf0090781 \- tracheal expression done by stainings towards
b-Galactosidase, which was detected from stage 15
Ref. FBrf0101899 \- tracheal expression done by stainings towards the Hdc
protein with a monoclonal Hdc antibody, which was detected from stage 14.
Fusion-6 is located at position 99F on the third chromosome.
Best regards,
Per Steneberg