Subject: Helping FlyBase: ADRC-10803
Dear Kweon,
We are currently curating the abstracts for the upcoming 42nd
(Washington, D.C.) Annual Drosophila Research Conference, for FlyBase.
I am writing in connection with your abstract:
'Cloning and Functional Characterization of a Neuropeptide F-like Receptor.'
You mention a gene that is new to FlyBase, at 76D5--76F2. Do you know
which of the Genome Project CG annotations your gene corresponds to?
All the CGs have corresponding gene records in FlyBase already and we
don't like to make duplicate records for what is actually the same gene
unless we can't avoid it. If your gene does not correspond to a CG
then perhaps you could tell me its map location, as this is valuable
information for the genome annotation project. Also, you don't name
your gene at all. Are you in a position to give a proper name to this
gene now.
Thank you for your help,
with best wishes,
Rachel.
Subject:  HelpingFlyBase:ADRC-10803 
you wrote to kweon yu asking about the abstract entitled: 'Cloning and
Functional Characterization of a Neiripeptide F-like Receptor' at position
76D5-76F2.
this is a gene we have been working on for several years...predating the
genome project. it was originally cloned by reduced stringency pcr looking
for other members of the g protein coupled receptor family. people
involved in the original cloning were gouping feng, renee venard, and me
(linda m. hall) all working in my lab. subsequent pharmacology and
expression characterization have involved peter evans (from cambridge, uk)
and kweon yu working in my lab here in sacramento.
in the genoome project, thos gene corresponds to CG7395. from its
structure we deduced that it was a peptide receptor, pharmacological
studies involving expression in xenopus oocytes shows that it best
activated by a neuropeptide F-like peptide. based on its pharmacology and
structure we propose to call it NPFR76F for neuropeptide F-like receptor
located at 76F.
we are finalizing a manuscript on this now.
let me know if you need additional information.
cheers,
Linda M. Hall, Ph.D.
Director of Research
Shriners Hospital for Children of Northern California
2425 Stockton Boulevard
Sacramento, CA 95817