Subject: FlyBase query
Dear Dr. Pelisson,
I am curating your paper for FlyBase:
Robert et al., 2001, Genetics 158(2): 701--713
1. There are a number of EST clots drawn in Figure 4.
They are:
clot#312
clot#1926
clot#10043
clot#6898
clot#9754
for each clot, could you give me the symbol of an EST that is in the
clot (if possible all the ESTs from each clot). e.g. LD12345 so that I
can unambiguously identify which ESTs are in the clot \- the clot numbers
on the BDGP site are not stable and the ESTs within each clot change
with time so I cannot be sure that if I look up the clot numbers on the
BDGP page whether the ESTs that are in the clot now are the same as
when you looked at the clot pages.
2. Does clot \#9754 in Figure 4. correspond to 'TO42' in GenBank
accession AF182444 ?
I look forward to hearing from you,
Gillian
\--------------------------------------------------------------
Gillian Millburn.
FlyBase (Cambridge),
\--------------------------------------------------------------
Subject: Re: FlyBase query
Dear Dr. Millburn,
...
clot#312 is represented by GH04962 (which matches the putative gene
'CG14476')
clot#6898 is represented by HL02945
clot#9754 is represented by two overlapping ESTs: GH24711 and GH08843. By
the way, concerning your second question , TO42 (AF213379) does indeed
match the ESTs of clot#9754, except for the 3' end , because of an
alternative polyadenylation.
You also ask for the names of ESTs expressed from the distal part of the
D1/D1' duplication (referred to as clots#1926&10043 in Fig.4): in fact,
as written at the bottom of page 707, these ESTs appeared to correspond to
alternative splicing variants (represented by GH25094 andLD08455) which
might originate from either (or both) of the duplicated transcription
units. However, since only the distal copy of the duplication was
sequenced, we could not address this question any further as we
tentatively did for the pair of clots \#6898&#9754 (see top of page 708).
...
Hoping all is now as clear as possible, I thank you for curating these
data.
Alain
\* PELISSON Alain
\* I.G.H.
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