Subject: DsRed Insertions
Personal communication from: Junko Kasuya and Linda Iverson, Beckman Research
Institute of the City of Hope
To: Bloomington Drosophila Stock Center
Subject: UAS-DsRed Insertions
Dated: 13 November 2000
Background: Stocks with these insertions were donated to the Bloomington
Stock Center. Mapping of the insertions to chromosome was done at the stock
center. Stocks to be added to the Bloomington collection contain the
following insertions:
Insertion Chromosome Phenotype of chromosome with insertion
P{UAS-AUG-DsRed}A 3 viable, fertile
P{UAS-AUG-DsRed}B 2 viable, homozygotes essentially sterile
P{UAS-AUG-DsRed}Ea 1 viable
P{UAS-AUG-DsRed}Eb 2 uncertain
P{UAS-AUG-DsRed}Ec 3 uncertain
Technical information on DsRed (Discosoma Red fluorescent protein) is
available at
http://www.clontech.com/archive/OCT99UPD/RFP.html
Information communicated:
'We generated several independent lines of Clontech's DsRed in the pUAST
vector and tested the flies using various Gal4 drivers to make certain that
they express DsRed in the correct tissue-specific pattern. We also tested with
GFP driven expression and there does not seem to be any problem detecting both
GFP and DsRED in the same fly.'