Gene or accession: CG15137
Release: 1
Gene annotation error
Gene CG15137 should be split.
Comments: http://cpmcnet.columbia.edu/dept/neurobeh/axel/gr.html
(being curated as Scott and Axel, 2001.4.25, personal communication to FlyBase)
describes 2 gustatory receptor (Gr) genes: Gr36B.2 and Gr36B.3, both of which
overlap somewhat with CG15137, suggesting that CG15137 may need splitting into
2 genes, or Gr36B.2 and Gr36B.3 may need merging (the alignment
of Gr36B.2 with CG15137 is not that great, so I'm not sure what needs
to happen).
alignments of the 2 Gr genes to CG15137 follow in e-mail to flybase-updates
Gillian
Subject: CG15137 alignments (for FlyBase error report)
here are alignments
BLASTP of GR against predicted proteins:
Query= Gr36B2 , 390 bases, A460EF58 checksum.
(390 letters)
Database: aa_gadfly.dros
14,080 sequences; 6,850,524 total letters.
Searching....10....20....30....40....50....60....70....80....90....100% done
Smallest
Sum
High Probability
Sequences producing High-scoring Segment Pairs: Score P(N) N
CG15137|FBan0015137|CT35034|FBan0015137  last_updated:000321  440 2.0e-50 2
>CG15137|FBan0015137|CT35034|FBan0015137  last_updated:000321 
Length = 361
Score = 440 (154.9 bits), Expect = 2.0e-50, Sum P(2) = 2.0e-50
Identities = 82/201 (40%), Positives = 132/201 (65%)
Query: 90 LITVLNRWLQRGQMMQLVKDVIRLYMINPQLKSMIRWGILLKAFISFAIELLQVTLSVDA 149
L T++ RW QR \+MM L V+R+Y+ PQ++ M RWGIL K \+ LQ+ \+ \+ A
Sbjct: 131 LFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQMAMVLSA 190
Query: 150 LDRQGTAEMMGLLVKLCVSFIMNLAISQHFLVILLIRAQYRIMNAKLRMVIEESRRLSFL 209
\+ \+ \+GL \++ \+ I+N+A+ Q \+++L \+R Q++++N \+LR VI+E++ L
Sbjct: 191 MGSVDSQFYLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKDLLLS 250
Query: 210 QLRNGAFMTRCCYLSDQLEDIGEVQSQLQSMVGQLDEVFGMQGLMAYSEYYLSIVGTSYM 269
G FMT+CC L+DQ+E+I \+QSQLQ+++ Q++EVFG+QG M Y YYLS VGT Y+
Sbjct: 251 PRHQGVFMTKCCSLADQIENIARIQSQLQTIMNQMEEVFGIQGAMTYGGYYLSSVGTCYL 310
Query: 270 SYSIYKYGPHNLKLSAKTSII 290
\+YSI K+G NL \++ T I+
Sbjct: 311 AYSILKHGYENLSMTLSTVIL 331
Score = 281 (98.9 bits), Expect = 4.7e-26, P = 4.7e-26
Identities = 60/81 (74%), Positives = 66/81 (81%)
Query: 260 YLSIVGTSYMSYSIYKYGPHNLKLSAKTSIIVCILITLFYLDALVNCNNMLRVLDHHKDF 319
\+L \+ \++M+ Y L+ AKTSIIVCILITLFYLDALVNCNNMLRVLDHHKDF
Sbjct: 17 FLQLRNGAFMTRCCYL--SDQLEDIAKTSIIVCILITLFYLDALVNCNNMLRVLDHHKDF 74
Query: 320 LGLLEERTVFASSLDIRLEES 340
LGLLEERTVFASSLDIRLEES
Sbjct: 75 LGLLEERTVFASSLDIRLEES 95
Score = 200 (70.4 bits), Expect = 5.7e-15, P = 5.7e-15
Identities = 42/76 (55%), Positives = 53/76 (69%)
Query: 192 MNAKLRMVIEESRRLSFLQLRNGAFMTRCCYLSDQLEDIGEVQSQLQSMVG--QLDEVFG 249
MNAKLRMVIEESRRLSFLQLRNGAFMTRCCYLSDQLEDI \+ \+ \++ LD \+
Sbjct: 1 MNAKLRMVIEESRRLSFLQLRNGAFMTRCCYLSDQLEDIAKTSIIVCILITLFYLDALVN 60
Query: 250 MQGLMAYSEYYLSIVG 265
\++ \+++ \+G
Sbjct: 61 CNNMLRVLDHHKDFLG 76
Score = 88 (31.0 bits), Expect = 2.0e-50, Sum P(2) = 2.0e-50
Identities = 19/34 (55%), Positives = 23/34 (67%)
Query: 1 MVDWVVLLLKAVHIYCYLIGLSNFEFDCRTGRVF 34
\++D LL AV+ Y IGLSNFEFD TGR+F
Sbjct: 99 IMDLESFLLGAVYYYGLFIGLSNFEFDWNTGRLF 132
Query= Gr36B3 , 344 bases, CF996511 checksum.
(344 letters)
Database: aa_gadfly.dros
14,080 sequences; 6,850,524 total letters.
Searching....10....20....30....40....50....60....70....80....90....100% done
Smallest
Sum
High Probability
Sequences producing High-scoring Segment Pairs: Score P(N) N
CG15137|FBan0015137|CT35034|FBan0015137  last_updated:000321  1053 7.3e-108 1
>CG15137|FBan0015137|CT35034|FBan0015137  last_updated:000321 
Length = 361
Score = 1053 (370.7 bits), Expect = 7.3e-108, P = 7.3e-108
Identities = 212/228 (92%), Positives = 215/228 (94%)
Query: 89 TG-LFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQMAMV 147
TG LFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQMAMV
Sbjct: 128 TGRLFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQMAMV 187
Query: 148 LSAMGSRVDSQFYLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKD 207
LSAMGS VDSQFYLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKD
Sbjct: 188 LSAMGS-VDSQFYLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKD 246
Query: 208 LLLSPRHQGVFMTKCCSLADQIENIARIQSQLQTIMNQMEEVFGIQGAMTYGGYYLSSVG 267
LLLSPRHQGVFMTKCCSLADQIENIARIQSQLQTIMNQMEEVFGIQGAMTYGGYYLSSVG
Sbjct: 247 LLLSPRHQGVFMTKCCSLADQIENIARIQSQLQTIMNQMEEVFGIQGAMTYGGYYLSSVG 306
Query: 268 TCYLAYSILKHGYENLSMTLSTVILAYSW-----CFFYYLDGMLNLSVM 311
TCYLAYSILKHGYENLSMTLSTVILAYSW C+ Y G \+L+ M
Sbjct: 307 TCYLAYSILKHGYENLSMTLSTVILAYSWMTTGKCYKYSGSGQYSLAWM 355