Gene or accession: CG14988 Release: 1 Gene annotation error Gene CG14988 should be split. Comments: CG14988 may need splitting in 2. http://cpmcnet.columbia.edu/dept/neurobeh/axel/gr.html (being curated as Scott and Axel, 2001.4.25, personal communication to FlyBase) describe 2 gustatory receptor (Gr) genes: 'Gr64A2' and 'Gr64A3' Gr64A2 sequence matches the N terminal portion of CG14988. Gr64A3 sequences matches the C terminal portion of CG14988, suggesting either that the CG14988 annotation needs splitting in two, or that Gr64A2 and Gr64A3 need merging. alignments of the 2 Gr genes to CG14988 follow. Subject: CG14988 alignments (for FlyBase error report) here are alignments BLASTP of GR against predicted proteins: Query= Gr64A2 , 367 bases, E86E1572 checksum. (367 letters) Database: aa_gadfly.dros 14,080 sequences; 6,850,524 total letters. Searching....10....20....30....40....50....60....70....80....90....100% done Smallest Sum High Probability Sequences producing High-scoring Segment Pairs: Score P(N) N CG14988|FBan0014988|CT34839|FBan0014988 last_updated:000321 1822 2.4e-189 1 CG15779|FBan0015779|CT36038|FBan0015779 last_updated:000321 666 7.5e-67 1 CG14986|FBan0014986|CT34837|FBan0014986 last_updated:000321 466 2.3e-44 1 CG14987|FBan0014987|CT34838|FBan0014987 last_updated:000321 129 2.3e-16 2 CG13888|FBan0013888|CT33421|FBan0013888 last_updated:000321 152 9.0e-10 1 >CG14988|FBan0014988|CT34839|FBan0014988 last_updated:000321 Length = 817 Score = 1822 (641.4 bits), Expect = 2.4e-189, P = 2.4e-189 Identities = 353/367 (96%), Positives = 353/367 (96%) Query: 1 MPVRKVSSKFAEDLTFTWFSVRSYYALVTILFFGVSSGYMVAFVTSVSFNFDSVETLVFY 60 MPVRKVSSKFAEDLTFTWFSVRSYYALVTILFFGVSSGYMVAFVTSVSFNFDSVETLVFY Sbjct: 1 MPVRKVSSKFAEDLTFTWFSVRSYYALVTILFFGVSSGYMVAFVTSVSFNFDSVETLVFY 60 Query: 61 LSIFLISLSFFQLARKWPEIAQSWQLVEAKLPPLKLPKERRSLAQHINMITIVATTCSLV 120 LSIFLISLSFFQLARKWPEIAQSWQLVEAKLPPLKLPKERRSLAQHINMITIVATTCSLV Sbjct: 61 LSIFLISLSFFQLARKWPEIAQSWQLVEAKLPPLKLPKERRSLAQHINMITIVATTCSLV 120 Query: 121 EHIMSMLSMGYYVNSCPRWPDRPIDXXXXXXXXXXXXXXDYTRFLGIVGKVVNVLSTFAW 180 EHIMSMLSMGYYVNSCPRWPDRPID DYTRFLGIVGKVVNVLSTFAW Sbjct: 121 EHIMSMLSMGYYVNSCPRWPDRPIDSFLYLSFSSVFYFVDYTRFLGIVGKVVNVLSTFAW 180 Query: 181 NFNDIFVMAVSVALAARFRQLNDYMMREARLPTTVDYWMQCRINFRNLCKLCEEVDDAIS 240 NFNDIFVMAVSVALAARFRQLNDYMMREARLPTTVDYWMQCRINFRNLCKLCEEVDDAIS Sbjct: 181 NFNDIFVMAVSVALAARFRQLNDYMMREARLPTTVDYWMQCRINFRNLCKLCEEVDDAIS 240 Query: 241 TITLLCFSNNLYFICGKILKSMQAKPSIWHALYFWFSLVYLLGRTLILSLYSSSINDESK 300 TITLLCFSNNLYFICGKILKSMQAKPSIWHALYFWFSLVYLLGRTLILSLYSSSINDESK Sbjct: 241 TITLLCFSNNLYFICGKILKSMQAKPSIWHALYFWFSLVYLLGRTLILSLYSSSINDESK 300 Query: 301 RPLVIFRLVPREYWCDELKRFSEEVQMDNVALTGMKFFRLTRGVVISVAGTIVTYELILL 360 RPLVIFRLVPREYWCDELKRFSEEVQMDNVALTGMKFFRLTRGVVISVAGTIVTYELILL Sbjct: 301 RPLVIFRLVPREYWCDELKRFSEEVQMDNVALTGMKFFRLTRGVVISVAGTIVTYELILL 360 Query: 361 QFNGEEK 367 QFNGEEK Sbjct: 361 QFNGEEK 367 BLASTP of GR against predicted proteins: Query= Gr64A3 , 409 bases, EAD6D3F1 checksum. (409 letters) Database: aa_gadfly.dros 14,080 sequences; 6,850,524 total letters. Searching....10....20....30....40....50....60....70....80....90....100% done Smallest Sum High Probability Sequences producing High-scoring Segment Pairs: Score P(N) N CG14988|FBan0014988|CT34839|FBan0014988 last_updated:000321 2066 3.3e-215 1 CG15779|FBan0015779|CT36038|FBan0015779 last_updated:000321 904 4.5e-92 1 CG14986|FBan0014986|CT34837|FBan0014986 last_updated:000321 578 2.4e-56 1 CG14987|FBan0014987|CT34838|FBan0014987 last_updated:000321 187 1.1e-25 2 CG13888|FBan0013888|CT33421|FBan0013888 last_updated:000321 194 1.2e-14 1 >CG14988|FBan0014988|CT34839|FBan0014988 last_updated:000321 Length = 817 Score = 2066 (727.3 bits), Expect = 3.3e-215, P = 3.3e-215 Identities = 397/409 (97%), Positives = 397/409 (97%) Query: 1 MELSRSDKEAFLSDGSFHQAVGRVLLVAEFFAMMPVKGVTGKHPSDLSFSWRNIRTCFSL 60 MELSRSDKEAFLSDGSFHQAVGRVLLVAEFFAMMPVKGVTGKHPSDLSFSWRNIRTCFSL Sbjct: 409 MELSRSDKEAFLSDGSFHQAVGRVLLVAEFFAMMPVKGVTGKHPSDLSFSWRNIRTCFSL 468 Query: 61 LFIASSLANFGLSLFKVLNNPISFNSIKPIIFRGSXXXXXXXXXXXXRQWPQLMMYWHTV 120 LFIASSLANFGLSLFKVLNNPISFNSIKPIIFRGS RQWPQLMMYWHTV Sbjct: 469 LFIASSLANFGLSLFKVLNNPISFNSIKPIIFRGSVLLVLIVALNLARQWPQLMMYWHTV 528 Query: 121 EKDLPQYKTQLTKWKMGHTISMVMLLGMMLSFAEHILSMVSAINYASFCNRTADPIQNYF 180 EKDLPQYKTQLTKWKMGHTISMVMLLGMMLSFAEHILSMVSAINYASFCNRTADPIQNYF Sbjct: 529 EKDLPQYKTQLTKWKMGHTISMVMLLGMMLSFAEHILSMVSAINYASFCNRTADPIQNYF 588 Query: 181 LRTNDEIFFVTSYSTTLALWGKFQNVFSTFIWNYMDLFVMIVSIGLASKFRQLNDDLRNF 240 LRTNDEIFFVTSYSTTLALWGKFQNVFSTFIWNYMDLFVMIVSIGLASKFRQLNDDLRNF Sbjct: 589 LRTNDEIFFVTSYSTTLALWGKFQNVFSTFIWNYMDLFVMIVSIGLASKFRQLNDDLRNF 648 Query: 241 KGMNMAPSYWSERRIQYRNICILCDKMDDAISLITMVSFSNNLYFICVQLLRSLNTMPSV 300 KGMNMAPSYWSERRIQYRNICILCDKMDDAISLITMVSFSNNLYFICVQLLRSLNTMPSV Sbjct: 649 KGMNMAPSYWSERRIQYRNICILCDKMDDAISLITMVSFSNNLYFICVQLLRSLNTMPSV 708 Query: 301 AHAVYFYFSLIFLIGRTLAVSLYSSSVHDESRLTLRYLRCVPKESWCPEVKRFTEEVISD 360 AHAVYFYFSLIFLIGRTLAVSLYSSSVHDESRLTLRYLRCVPKESWCPEVKRFTEEVISD Sbjct: 709 AHAVYFYFSLIFLIGRTLAVSLYSSSVHDESRLTLRYLRCVPKESWCPEVKRFTEEVISD 768 Query: 361 EVALTGMKFFHLTRKLVLSVAGTIVTYELVLIQFHEDNDLWDCDQSYYS 409 EVALTGMKFFHLTRKLVLSVAGTIVTYELVLIQFHEDNDLWDCDQSYYS Sbjct: 769 EVALTGMKFFHLTRKLVLSVAGTIVTYELVLIQFHEDNDLWDCDQSYYS 817
