Subject: BDGP gene annotation
Dear Sir/Madam,
I have recently been looking at predicted genes for g-protein coupled
receptors on the sequence database and believe I have found a mistake in the
annotation of one gene. I thought I should bring this to your attention,
although it is a bit complicated to explain through the 'fix annotation'
option.
I believe that the DNA sequence for the protein receptor NPFR76F has
actually been wrongly divided as two CG's, CG7395 and CG18729 (CG18639). It
also requires a small portion of genomic sequence to be included between the
two CG's as well as full read through to the stop codon contained at the end
of CG18729 .
A full length ORF can be gained by reading from the start codon in CG7395
(nt 702 of cDNA) through the genomic sequence (AE003514 250814-250921) and
into the coding sequence of CG18729 to the termination codon. This ORF
sequence translated gives a matching amino acid sequence to NPFR76F.
Yours faithfully,
<br><br><br>Jon Radford
Dow/Davies Lab
Molecular Genetics
Anderson College
IBLS, Univ of Glasgow